Vovkun T V, Yanchuk P I, Shtanova L Y, Vesеlskyу S P, Shalamaу A S
Fiziol Zh (1994). 2016;62(3):30-8. doi: 10.15407/fz62.03.030.
In acute experiments on rats with cannulated bile duct we studied the effect of Corvitin, water-soluble analogue of quercetin, on secretion of bile. Intraportal administration of the test compound at doses of 2,5; 5 and 10 mg/kg resulted in a significant increase in the volume of secreted bile by 20,9, 31,2 and 20,4%, respectively, as compared with the control. Using the method of thin layer chromatography it was established the mild stimulating effect of Corvitin on the processes of bile acids conjugation with taurine and glycine, especially when administered at a dose of 5 mg/kg. This flavonoid did not affect the concentration of glycocholic acid, however increased the content of glycochenodeoxycholic and glycodeoxycholic acids in the mixture between 15 to 35,1%. Regarding free bile acids, the concentration of cholic acid, chenodeoxycholic and deoxycholic acids in the mixture was increased significantly relative to control only after Corvitin application at dose 10 mg/ kg. In the first case – from 17,9 to 29,8%, in the second – from 25 to 65,4%. At the dose of 5 mg/kg, Corvitin significantly increased the ratio of bile cholates conjugation (maximum by 23,2%), whereas 10 mg/kg of the drug decreased this index by 27,0%. After administration of Corvitin, the hydroxylation ratio in all experimental groups differed little from the control: at the dose of 5 and 10 mg/kg this parameter decreased by 14%. Thus, Corvitin modulates exocrine function of the liver, causing an increase in bile secretion and concentration of different cholates, dose-dependently increasing or decreasing the effectiveness of multienzyme systems providing processes of bile acids conjugation in rats.
在对胆管插管的大鼠进行的急性实验中，我们研究了槲皮素的水溶性类似物Corvitin对胆汁分泌的影响。以2.5、5和10mg/kg的剂量门静脉内注射受试化合物，与对照组相比，分泌胆汁的量分别显著增加了20.9%、31.2%和20.4%。采用薄层色谱法确定，Corvitin对胆汁酸与牛磺酸和甘氨酸结合过程有温和的刺激作用，尤其是在以5mg/kg的剂量给药时。这种类黄酮不影响甘氨胆酸的浓度，但使混合物中甘氨鹅去氧胆酸和甘氨脱氧胆酸的含量增加了15%至35.1%。关于游离胆汁酸，仅在以10mg/kg的剂量应用Corvitin后，混合物中胆酸、鹅去氧胆酸和脱氧胆酸的浓度相对于对照组才显著增加。在第一种情况下，从17.9%增加到29.8%，在第二种情况下，从25%增加到65.4%。在5mg/kg的剂量下，Corvitin显著增加了胆汁胆酸盐结合率（最高增加23.2%），而10mg/kg的药物使该指标降低了27.0%。给予Corvitin后，所有实验组的羟基化率与对照组差异不大：在5和10mg/kg的剂量下，该参数降低了14%。因此，Corvitin调节肝脏的外分泌功能，导致胆汁分泌增加和不同胆酸盐浓度升高，剂量依赖性地增加或降低大鼠体内提供胆汁酸结合过程的多酶系统的有效性。
