Horenko Z A, Karabovs'ka L S, Vasheka I P, Vesel's'kyĭ S P
Fiziol Zh (1994). 2011;57(3):40-8.
The influence of calcitonin on the choleresis level and bile acids spectrum was investigated in acute experiments on the rats with common biliary duct cannulated. We determined the intensity of bile secretion during 3 hours and quantitative content of conjugated and free bile acids using thin-layer chromatography. It was shown that intramuscular introduction of synthetic salmon calcitonin (200 ng/kg) increases the secreted bile volume and the content of conjugated bile acids in the bile. The administration of salmon calcitonin resulted in increase of bile volume (+17.4% (P<0.05)), taurotrihydroxycholates (+58,3% (P<.001)), taurodihydroxycholates (+68.5% (P<0.01)), glycodihydroxycholates (+120.8% (P<0.01)), while the total content of free bile acids was not significantly altered as compared with control. At the same time, under the hormone influence we observed bile acid spectrum redistribution and a part of conjugated cholates increased against a decreasing of free bile acids part. The present results suggest that the salmon calcitonin intensifies the processes of conjugation of bile acids with amino acids taurine and glycine in hepatocytes and canalicular secretion that results in improvement of solubilization properties of the bile, ability of the bile to hold cholesterol in solute state and prevent the formation of calculi in biliary tracts.
在对胆总管插管大鼠进行的急性实验中，研究了降钙素对胆汁分泌水平和胆汁酸谱的影响。我们通过薄层色谱法测定了3小时内胆汁分泌的强度以及结合型和游离型胆汁酸的定量含量。结果表明，肌肉注射合成鲑鱼降钙素（200 ng/kg）可增加胆汁分泌量以及胆汁中结合型胆汁酸的含量。给予鲑鱼降钙素后，胆汁量增加了17.4%（P<0.05），牛磺三羟基胆酸盐增加了58.3%（P<0.001），牛磺二羟基胆酸盐增加了68.5%（P<0.01），甘氨二羟基胆酸盐增加了120.8%（P<0.01），而游离胆汁酸的总含量与对照组相比无显著变化。同时，在激素影响下，我们观察到胆汁酸谱发生重新分布，一部分结合型胆酸盐增加，而游离胆汁酸部分减少。目前的结果表明，鲑鱼降钙素可增强肝细胞和胆小管分泌中胆汁酸与氨基酸牛磺酸和甘氨酸的结合过程，从而改善胆汁的溶解特性、胆汁将胆固醇保持在溶质状态的能力，并防止胆道结石的形成。
