使用扫描电子显微镜评估钛激光处理表面上的纤维蛋白凝块附着情况。

Evaluation of Fibrin Clot Attachment on Titanium Laser-Conditioned Surface Using Scanning Electron Microscopy.

作者信息

Sinjari Bruna, Traini Tonino, Caputi Sergio, Mortellaro Carmen, Scarano Antonio

机构信息

Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, Chieti.

Oral Surgery Unit, University of Piedmont, Novara.

出版信息

J Craniofac Surg. 2018 Nov;29(8):2277-2281. doi: 10.1097/SCS.0000000000004519.

DOI:10.1097/SCS.0000000000004519

PMID:29570521

Abstract

OBJECTIVES

The study aimed to evaluate the effects of different titanium surface treatments on blood clot extension (bce).

METHODS

A total of 54 titanium disks with machined surface (M), laser-conditioned surface (L), and grit-blasted surface (S) were used in the present study. The surface characteristics such as contact angles and the microroughness were determined on each group (n = 4). To evaluate the bce, 0.1 mL of human blood was dropped onto the surface of each specimen and left for 7 minutes at room temperature. After fixation, dehydration, and gold sputtering treatments, the specimens were observed under scanning electron microscope. The bce values were expressed as percentage of specimen surface covered by blood clot.

RESULTS

The surface roughness (Ra ± standard deviation [SD]) was 0.75 ± 0.02 μm for M, 0.25 ± 0.02 μm for L, and 1.30 ± 0.03 μm for S. The contact angles measured in static conditions (WCA ± SD) were 71 ± 5.4° for M, 107 ± 6.6° for L, and 91 ± 7.2° for S. Regarding the bce (bce ± SD) of M samples (65.5 ± 4.3%) was statistically lower compared with both L (83.4 ± 5.1%) and S samples (72.4 ± 4.7%) (P < 0.05). Meanwhile, the L group showed the higher bce value.

CONCLUSION

The present results suggest that the laser-conditioned surface may increase the wettability and bce.

摘要

目的

本研究旨在评估不同钛表面处理对血凝块扩展（bce）的影响。

方法

本研究共使用了54个具有机械加工表面（M）、激光处理表面（L）和喷砂表面（S）的钛盘。测定每组（n = 4）的表面特性，如接触角和微观粗糙度。为了评估bce，将0.1 mL人血滴在每个标本表面，并在室温下放置7分钟。经过固定、脱水和喷金处理后，在扫描电子显微镜下观察标本。bce值以血凝块覆盖的标本表面百分比表示。

结果

M组的表面粗糙度（Ra±标准差[SD]）为0.75±0.02μm，L组为0.25±0.02μm，S组为1.30±0.03μm。静态条件下测得的接触角（WCA±SD），M组为71±5.4°，L组为107±6.6°，S组为91±7.2°。关于bce，M组样本的bce（bce±SD）为65.5±4.3%，与L组（83.4±5.1%）和S组（72.4±4.7%）相比，在统计学上较低（P<0.05）。同时，L组显示出较高的bce值。