Grizanova Ekaterina V, Semenova Alexandra D, Komarov Denis A, Chertkova Ekaterina A, Slepneva Irina A, Dubovskiy Ivan M
Novosibirsk State Agrarian University, Novosibirsk, Russia.
Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Siberian Branch, Novosibirsk, Russia.
Arch Insect Biochem Physiol. 2018 Aug;98(4):e21460. doi: 10.1002/arch.21460. Epub 2018 Mar 23.
The lipid peroxidation process in hemocytes, activities of phenoloxidase and key enzymatic antioxidants (superoxide dismutase, glutathione-S-transferase, catalase) and nonenzymatic antioxidants (thiols, ascorbate) in hemolymph of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) were studied during the encapsulation process of nylon implants. It has been established that as soon as 15 min after piercing a cuticle with the implant, a capsule is formed on its surface. Active melanization of the capsule has been shown to last for 4 h. During the first hours after incorporating the implant, an increase in phenoloxidase activity and lipid peroxidation in the insect hemocytes has been revealed. Adhesion and degranulation on the surface of foreign object lead to the depletion of total hemocytes count (THC). Our results indicated that thiols and ascorbate molecules take part in the immediate antioxidant response, during later stages of encapsulation process hemolymph glutathione-S-transferase detoxifies and protects insect organism thereby restoring the internal redox balance. We suggest that nonenzymatic and enzymatic antioxidants of hemolymph plasma play a key role in the maintenance of redox balance during encapsulation of foreign targets.
在大蜡螟（Galleria mellonella L.，鳞翅目：螟蛾科）尼龙植入物的包囊化过程中，研究了血细胞中的脂质过氧化过程、酚氧化酶以及关键酶促抗氧化剂（超氧化物歧化酶、谷胱甘肽 - S - 转移酶、过氧化氢酶）和血淋巴中的非酶促抗氧化剂（硫醇、抗坏血酸）的活性。已确定在用植入物刺穿表皮后15分钟，其表面就会形成一个包囊。已表明包囊的活性黑化持续4小时。在植入物植入后的最初几个小时内，已发现昆虫血细胞中的酚氧化酶活性和脂质过氧化增加。异物表面的黏附和解粒作用导致总血细胞计数（THC）减少。我们的结果表明，硫醇和抗坏血酸分子参与即时抗氧化反应，在包囊化过程的后期，血淋巴谷胱甘肽 - S - 转移酶解毒并保护昆虫机体，从而恢复内部氧化还原平衡。我们认为，血淋巴中的非酶促和酶促抗氧化剂在对外源靶标的包囊化过程中维持氧化还原平衡方面起着关键作用。
