明胶海绵上功能性人淋巴组织的组织培养及HIV感染
Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam.
作者信息
Introini Andrea, Fitzgerald Wendy, Vanpouille Christophe, Margolis Leonid
机构信息
Karolinska Institute, Stockholm, Sweden.
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2018;1760:187-197. doi: 10.1007/978-1-4939-7745-1_17.
Gelfoam histoculture provides a valuable tool for experimental studies of normal and pathological tissue physiology. It allows us to understand cell-cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with HIV and maintain them in Gelfoam histoculture at the liquid-air interface. The Gelfoam histocultures with human immunodeficiency virus (HIV) type 1-infected tissues have been used to further understand the biology of early HIV-1 pathogenesis, as well as a novel ex vivo platform to test the efficacy and toxicity of antiviral drugs.
明胶海绵组织培养为正常和病理组织生理学的实验研究提供了一种有价值的工具。它使我们能够通过反映细胞在身体组织内的原始空间关系来理解细胞间相互作用。明胶海绵组织培养可用于在体外模拟体内条件来建立宿主 - 病原体相互作用模型。在本章中，我们描述了一种处理和用HIV感染淋巴组织外植体并将其维持在液 - 气界面的明胶海绵组织培养中的方案。感染了1型人类免疫缺陷病毒（HIV）的组织的明胶海绵组织培养已被用于进一步了解早期HIV - 1发病机制的生物学特性，以及作为一个测试抗病毒药物疗效和毒性的新型离体平台。
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