Companion Animal Clinic (Medicine Unit), School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
PLoS One. 2021 Jan 20;16(1):e0245713. doi: 10.1371/journal.pone.0245713. eCollection 2021.
Canine inflammatory bowel disease (IBD) is a group of chronic gastrointestinal disorders, the pathogenesis of which remains elusive, but it possibly involves the interaction of the intestinal immune system with luminal microbiota and food-derived antigens. Mucosal cytokines profiles in canine IBD have been investigated mainly in small intestinal disease, while data on cytokine profiles in large intestinal IBD are limited. The objective of this study was to measure colonic mucosal and cytobrush sample messenger (m)RNA expression of interleukin (IL)-1β, IL-2, IL-12p40, IL-23p19, tumor necrosis factor-alpha (TNF-α) and chemokine C-C motif ligand (CCL28) in dogs with IBD and healthy controls using quantitative real-time polymerase chain reaction (PCR), and assess their correlation with clinical disease activity, endoscopic and histopathologic score. Dogs with IBD had a significantly increased mRNA expression of IL-1β, IL-23p19 and CCL28 in the colonic mucosa, compared to healthy controls. None of the selected cytokines had significantly different mRNA expression in the colonic cytobrush samples between the two groups or between the colonic mucosa and cytobrush samples of dogs with IBD. Finally, there was a statistically significant correlation of clinical disease activity with endoscopic activity score and fibrosis and atrophy of the colonic mucosa in dogs with large intestinal IBD. IL-1β, IL-23p19 and CCL28 could play a role in the pathogenesis of canine large intestinal IBD. Colonic cytokine expression does not correlate with clinical disease activity and/or endoscopic score. However, clinical signs reflect the severity of endoscopic lesions.
犬炎症性肠病（IBD）是一组慢性胃肠道疾病，其发病机制尚不清楚，但可能涉及肠道免疫系统与腔微生物群和食物来源抗原的相互作用。犬 IBD 的黏膜细胞因子谱主要在小肠疾病中进行了研究，而关于大肠 IBD 中细胞因子谱的数据有限。本研究的目的是使用定量实时聚合酶链反应（PCR）测量 IBD 犬和健康对照犬的结肠黏膜和细胞刷样本信使（m）RNA 表达白细胞介素（IL）-1β、IL-2、IL-12p40、IL-23p19、肿瘤坏死因子-α（TNF-α）和趋化因子 C-C 基序配体（CCL28），并评估它们与临床疾病活动、内镜和组织病理学评分的相关性。与健康对照组相比，IBD 犬的结肠黏膜中 IL-1β、IL-23p19 和 CCL28 的 mRNA 表达显著增加。两组之间或 IBD 犬的结肠细胞刷样本与结肠黏膜样本之间，所选细胞因子的 mRNA 表达均无显著差异。最后，在患有大肠 IBD 的犬中，临床疾病活动与内镜活动评分以及结肠黏膜纤维化和萎缩之间存在统计学上显著的相关性。IL-1β、IL-23p19 和 CCL28 可能在犬大肠 IBD 的发病机制中起作用。结肠细胞因子表达与临床疾病活动和/或内镜评分不相关。然而，临床症状反映了内镜病变的严重程度。
