Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Hermann-von-Helmholtz Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Institute of Organic Chemistry, Karlsruhe Institute of Technology, 76131, Karlsruhe, Germany.
Adv Mater. 2018 May;30(20):e1706111. doi: 10.1002/adma.201706111. Epub 2018 Mar 23.
High-throughput screening of live cells and chemical reactions in isolated droplets is an important and growing method in areas ranging from studies of gene functions and the search for new drug candidates, to performing combinatorial chemical reactions. Compared with microfluidics and well plates, the facile fabrication, high density, and open structure endow droplet microarrays on planar surfaces with great potential in the development of next-generation miniaturized platforms for high-throughput applications. Surfaces with special wettability have served as substrates to generate and/or address droplets microarrays. Here, the formation of droplet microarrays with designed geometry on chemically prepatterned surfaces is briefly described and some of the newer and emerging applications of these microarrays that are currently being explored are highlighted. Next, some of the available technologies used to add (bio-)chemical libraries to each droplet in parallel are introduced. Current challenges and future prospects that would benefit from using such droplet microarrays are also discussed.
高通量筛选活细胞和化学反应在分离液滴中是一个重要的和不断发展的方法在各个领域从基因功能的研究和寻找新的候选药物,执行组合化学反应。相比微流控和微孔板,简单的制造,高密度,和开放式结构赋予液滴微阵列在平面表面具有很大的潜力在开发下一代的小型化平台用于高通量应用。具有特殊润湿性的表面可用作基底来生成和/或解决液滴微阵列。在这里，简要描述了在化学预图案化表面上形成具有设计几何形状的液滴微阵列，并且突出了目前正在探索的这些微阵列的一些更新和新兴应用。接下来，介绍了一些可用于并行向每个液滴添加(生物)化学库的现有技术。还讨论了当前利用这种液滴微阵列所面临的挑战和未来的前景。
