Institut Ciencia Materials Barcelona (ICMAB-CSIC)-CIBER-BBN, Campus Universitari de Bellaterra, 08193, Cerdanyola, Spain.
Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università di Parma, Parco Area Delle Scienze 17/A, 43124, Parma, Italy.
Small. 2018 Apr;14(16):e1703851. doi: 10.1002/smll.201703851. Epub 2018 Mar 24.
A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI), 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indodicarbocyanine perchlorate (DiD), and 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO -based technology, show excellent colloidal- and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI-loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.
一种新型荧光有机纳米粒子（FONs）是使用 quatsomes（QSs）获得的，QSs 是一类纳米囊泡，被提议作为商业亲脂性碳菁染料（1,1'-二辛基-3,3,3',3'-四甲基吲哚碳菁高氯酸盐（DiI）、1,1'-二辛基-3,3,3',3'-四甲基吲哚二碳菁高氯酸盐（DiD）和 1,1'-二辛基-3,3,3',3'-四甲基吲哚三碳菁碘化物（DiR））在水介质中纳米结构化的支架。通过 CO 基技术制备的所得 FONs 表现出优异的胶体和光稳定性，优于染料的其他纳米制剂，并改善了荧光团在水中的光学性质。分子动力学模拟提供了染料在膜内位置的原子级图像。通过对体外和细胞中负载 DiI 的囊泡进行超分辨率显微镜成像，证明了 QSs 在生物成像中的潜力。因此，荧光 QSs 构成了一种有吸引力的用于生物成像应用的纳米材料。
