Ranjkesh Bahram, Isidor Flemming, Kraft David Christian Evar, Løvschall Henrik
Department of Dentistry and Oral Health, Aarhus University.
J Oral Sci. 2018;60(1):82-88. doi: 10.2334/josnusd.16-0751.
A novel fast-setting calcium silicate cement with fluoride (CSC) has been developed for potential application in tooth crowns. This study compared the cytotoxicity of CSC compositions and a variety of dental materials. We tested CSC compositions (Protooth), MTA, Biodentine, Ketac Molar, Fuji II LC, Vitrebond, DeTrey Zinc, Dycal, and IRM, DMEM (negative control) and 1% NaOCl (positive control). After setting of cements for 24 h, specimens were immersed in DMEM for 24 h to obtain material elutes. The elutes were serially diluted in serum-free DMEM to obtain three dilutions. L929 mouse fibroblast cells (1 × 10 cells per well) were treated for 24 h with elute dilutions (n = 3). Cytotoxicity was determined using methyl-thiazolyl-tetrazolium assay in triplicate. CSC compositions, MTA, and Biodentine showed no significant reduction in cell viability compared to DMEM. There was no significant difference in cell viability, at any of three dilutions, between CSC compositions and either MTA or Biodentine. Cytotoxicity was significantly lower for CSC compositions than for Vitrebond, DeTrey Zinc, Dycal, IRM, and 1% NaOCl, at all three dilutions, and undiluted Fuji II LC elute. In contrast to resin-modified glass ionomers, zinc phosphate cements, Dycal, and IRM, the CSC compositions showed no cytotoxic potential.
一种新型的含氟快凝硅酸钙水泥（CSC）已被开发用于牙冠的潜在应用。本研究比较了CSC组合物与多种牙科材料的细胞毒性。我们测试了CSC组合物（Protooth）、MTA、生物活性牙本质、Ketac Molar、富士II LC、Vitrebond、DeTrey锌、Dycal和IRM、DMEM（阴性对照）和1%次氯酸钠（阳性对照）。水泥凝固24小时后，将标本浸入DMEM中24小时以获得材料洗脱液。洗脱液在无血清DMEM中连续稀释以获得三种稀释度。用洗脱液稀释液处理L929小鼠成纤维细胞（每孔1×10个细胞）24小时（n = 3）。使用甲基噻唑基四氮唑法一式三份测定细胞毒性。与DMEM相比，CSC组合物、MTA和生物活性牙本质在细胞活力方面没有显著降低。在三种稀释度的任何一种下，CSC组合物与MTA或生物活性牙本质之间的细胞活力没有显著差异。在所有三种稀释度以及未稀释的富士II LC洗脱液中，CSC组合物的细胞毒性明显低于Vitrebond、DeTrey锌、Dycal、IRM和1%次氯酸钠。与树脂改性玻璃离子水门汀、磷酸锌水门汀、Dycal和IRM不同，CSC组合物没有细胞毒性潜力。
