Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal.
Institute of Biophysics, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.
Molecules. 2020 Sep 19;25(18):4297. doi: 10.3390/molecules25184297.
The sealers used for root canal treatment should be biocompatible for the peri-radicular tissues, to evaluate the cytotoxic effects of GuttaFlow bioseal sealer and to compare them with AH26 epoxy resin. Culture media were conditioned with the GuttaFlow bioseal and AH26 pellets. MDPC-23 odontoblast cell cultures were treated with conditioned medium and serial dilutions. To evaluate the metabolic activity and cellular viability, the MTT and SRB assays were performed. To determine the production of reactive oxygen species, the DHE and DCF-DA probes were used. Cell cycle and cell-death types were assessed by cytometry, and to evaluate the mineralization capacity, the Alizarin Red S coloration was used. Statistical analysis was performed using analysis of variance (ANOVA) when normality was found and Kruskal-Wallis on the opposite case. For the comparison with normality values, the Student -test was used. Cells exposed to the GuttaFlow bioseal conditioned medium maintained high metabolic activities, except at higher concentrations. Likewise, viability was maintained, but a significant decrease was observed after exposure to the highest concentration ( < 0.001), associated with cell death by late apoptosis and necrosis. When cell cultures were exposed to AH26, metabolic activity was highly compromised, resulting in cell death. An imbalance in the production of peroxides and superoxide anion was observed. GuttaFlow bioseal showed higher biocompatibility than AH26.
用于根管治疗的密封剂应与根尖周组织具有生物相容性，以评估 GuttaFlow 生物密封剂的细胞毒性作用，并将其与 AH26 环氧树脂进行比较。将 GuttaFlow 生物密封剂和 AH26 颗粒与培养基混合。用条件培养基和系列稀释液处理 MDPC-23 成牙本质细胞培养物。为了评估代谢活性和细胞活力，进行了 MTT 和 SRB 测定。为了确定活性氧的产生，使用 DHE 和 DCF-DA 探针。通过细胞计数评估细胞周期和细胞死亡类型，并通过茜素红 S 染色评估矿化能力。如果发现正态性，则使用方差分析（ANOVA）进行统计分析，否则使用 Kruskal-Wallis 检验。为了与正态性值进行比较，使用了学生 t 检验。暴露于 GuttaFlow 生物密封剂条件培养基的细胞保持高代谢活性，除了在较高浓度下。同样，细胞活力得以维持，但在暴露于最高浓度后观察到显著下降（<0.001），与晚期凋亡和坏死相关的细胞死亡有关。当细胞培养物暴露于 AH26 时，代谢活性受到严重损害，导致细胞死亡。观察到过氧化物和超氧阴离子产生的不平衡。GuttaFlow 生物密封剂的生物相容性优于 AH26。
