Chen Ning, Guo Chang-E, Chen Hongying, Chen Jianhua, Bi Xinning, Li Hongpin, Zhu Hongyu, Ma Pengkai, Zhang Yujie, Lin Hongying
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Biomed Chromatogr. 2018 Aug;32(8):e4248. doi: 10.1002/bmc.4248. Epub 2018 May 16.
Jiao-Tai-Wan (JTW) is a well-known traditional Chinese medicine prescription composed of Rhizoma Coptidis (RC) and Cortex Cinnamon (10:1, g/g). It has been used to treat insomnia in China for centuries. This study investigates the excretion properties of coptis alkaloids from RC and JTW in normal and insomniac rats, and it examines the compatibility mechanism for this prescription. A new liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of six alkaloids - berberine, epiberberine, coptisine, jatrorrhizine, palmatine and magnoflorine - in rat urine and feces. The normal and model rats were orally treated with RC and JTW powder at a dosage containing 3.0 g kg day RC once per day for 7 days. Briefly, the results showed that the cumulative amounts of urinary and fecal excretion of the six alkaloids were significantly different in the pathological condition, as well as in compatibility. In normal rats, the urinary and fecal excretion of coptis alkaloids, especially berberine, coptisine and palmatine, increased significantly in the JTW group compared with the RC group, while the urinary and fecal excretion of six alkaloids decreased in insomniac rats. These data suggested that pathological conditions might have a notable influence on the excretion of alkaloids in rats, and demonstrated that the compatibility could promote better therapeutic effects through the accumulation of alkaloids in the body. These results might explain the compatibility of JTW.
交泰丸（JTW）是一种著名的中药方剂，由黄连（RC）和肉桂（10:1，克/克）组成。几个世纪以来，它在中国一直被用于治疗失眠。本研究调查了正常大鼠和失眠大鼠中黄连生物碱从黄连及交泰丸中的排泄特性，并研究了该方剂的配伍机制。建立了一种新的液相色谱-串联质谱法，用于同时测定大鼠尿液和粪便中的六种生物碱——小檗碱、表小檗碱、黄连碱、药根碱、巴马汀和木兰碱。正常大鼠和模型大鼠每天口服一次含3.0 g·kg⁻¹黄连的交泰丸粉末，连续7天。简而言之，结果表明，六种生物碱的尿排泄和粪排泄累积量在病理状态以及配伍方面均存在显著差异。在正常大鼠中，与黄连组相比，交泰丸组黄连生物碱的尿排泄和粪排泄，尤其是小檗碱、黄连碱和巴马汀，显著增加，而在失眠大鼠中六种生物碱的尿排泄和粪排泄减少。这些数据表明，病理状态可能对大鼠生物碱的排泄有显著影响，并证明配伍可通过生物碱在体内的蓄积促进更好的治疗效果。这些结果可能解释了交泰丸的配伍原理。
