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[食指与接触者细菌ERIC-PCR指纹图谱的比较]

[Comparison of Bacteria ERIC-PCR Fingerprints of Index Fingers and Contactants].

作者信息

Liu Y T, Sun D M, Shi S P, Yang X

机构信息

East China University of Political Science and Law, Shanghai 200043, China.

Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.

出版信息

Fa Yi Xue Za Zhi. 2018 Feb;34(1):33-36. doi: 10.3969/j.issn.1004-5619.2018.01.007. Epub 2018 Feb 25.

DOI:10.3969/j.issn.1004-5619.2018.01.007
PMID:29577702
Abstract

OBJECTIVES

To explore the bacteria relevance between index fingers and contactant' surfaces (mobile phone touch screen and desktop of personal office table).

METHODS

Bacteria were collected from the index fingers, mobile phone touch screen and desktop of personal office table of 10 volunteers. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint was established by PCR amplification technique of metagenome.

RESULTS

There were 7 volunteers' ERIC-PCR fingerprints of index fingers matched that took from the mobile phone touch screens, and different from each other. There were 3 volunteers' ERIC-PCR fingerprints of index fingers matched that took from desk top of personal office table, and other 7 volunteers' ERIC-PCR fingerprints did not match perfectly with that took from desk top of personal office table, but had at least one similar band for both.

CONCLUSIONS

The bacteria on index finger shows individual specificity, which on mobile phone touching screen and personal desktop may be a new biological sample of forensic identification.

摘要

目的

探讨食指与接触物体表面(手机触摸屏和个人办公桌桌面)之间的细菌相关性。

方法

从10名志愿者的食指、手机触摸屏和个人办公桌桌面上采集细菌。通过宏基因组PCR扩增技术建立肠杆菌重复基因间共识(ERIC)-PCR指纹图谱。

结果

7名志愿者食指的ERIC-PCR指纹图谱与手机触摸屏上的指纹图谱匹配,且彼此不同。3名志愿者食指的ERIC-PCR指纹图谱与个人办公桌桌面上的指纹图谱匹配,另外7名志愿者食指的ERIC-PCR指纹图谱与个人办公桌桌面上的指纹图谱不完全匹配,但两者至少有一条相似条带。

结论

食指上的细菌具有个体特异性,手机触摸屏和个人桌面上的细菌可能是法医鉴定的新生物样本。

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