Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2018 May;118:247-250. doi: 10.1016/j.micpath.2018.03.034. Epub 2018 Mar 22.
The presence of Brucella melitensis and Brucella abortus genomes were investigated in the synovial fluid (SF) samples from 90 patients with rheumatoid arthritis (RA). DNA extraction and PCR assay were performed for simultaneous identification and discrimination of B. melitensis and B. abortus from the SF using three specific primers. After gel electrophoresis, the PCR products were confirmed by DNA sequencing. The cbg, omp31, manA, virB, and znuA virulence genes typing were performed by multiplex-PCR. Of the 90 samples, 14 were positive for B. melitensis (n = 9; 10%) and B. abortus (n = 5; 5.5%). The virulotyping of positive samples revealed the presence of all five virulence genes in B. melitensis. The virB, cbg, and om31 were detected in all five samples of B. abortus. In addition, zhuA and manA were detected in three (60%) and four (80%) samples, respectively, of the B. abortus-positive samples. Moreover, a total of 94.2% and 89.2% of the 14 positive samples were also found positive for manA and znuA, respectively. Our findings revealed that the Brucella spp. genomes can be detected in the SF of RA patients by the PCR-based method. We thus suggest that physicians should consider the Brucella spp. as indicators of potential RA for the timely diagnosis and treatment of RA.
我们调查了 90 例类风湿关节炎（RA）患者关节滑液（SF）样本中是否存在布鲁氏菌 melitensis 和布鲁氏菌 abortus 基因组。使用 3 种特异性引物，通过 DNA 提取和 PCR 检测，对 SF 中的布鲁氏菌 melitensis 和布鲁氏菌 abortus 进行同时鉴定和区分。凝胶电泳后，通过 DNA 测序对 PCR 产物进行确认。通过多重 PCR 对 cbg、omp31、manA、virB 和 znuA 毒力基因进行分型。90 个样本中，有 14 个为布鲁氏菌 melitensis（n=9；10%）和布鲁氏菌 abortus（n=5；5.5%）阳性。阳性样本的毒力分型显示，布鲁氏菌 melitensis 存在所有 5 种毒力基因。布鲁氏菌 abortus 的所有 5 个样本中均检测到了 virB、cbg 和 om31。此外，在布鲁氏菌 abortus 阳性样本中，zhuA 和 manA 分别在 3 个（60%）和 4 个（80%）样本中被检测到。此外，14 个阳性样本中有 94.2%和 89.2%也分别检测到 manA 和 znuA 阳性。我们的研究结果表明，PCR 方法可在 RA 患者的 SF 中检测到布鲁氏菌 spp. 基因组。因此，我们建议医生应将布鲁氏菌 spp. 作为潜在 RA 的指标，以进行 RA 的及时诊断和治疗。
