Centre de recherche en infectiologie, CHU de Québec-Université Laval, Quebec City, Quebec, Canada.
Integrative Parasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany.
Cell Microbiol. 2018 Aug;20(8):e12843. doi: 10.1111/cmi.12843. Epub 2018 Apr 24.
Compared with other eukaryotic cell types, malaria parasites appear to possess a more rudimentary Golgi apparatus being composed of dispersed, unstacked cis and trans-cisternae. Despite playing a central role in the secretory pathway of the parasite, few Plasmodium Golgi resident proteins have been characterised. We had previously identified a new Golgi resident protein of unknown function, which we had named Golgi Protein 1, and now show that it forms a complex with a previously uncharacterised transmembrane protein (Golgi Protein 2, GP2). The Golgi Protein complex localises to the cis-Golgi throughout the erythrocytic cycle and potentially also during the mosquito stages. Analysis of parasite strains where GP1 expression is conditionally repressed and/or the GP2 gene is inactivated reveals that though the Golgi protein complex is not essential at any stage of the parasite life cycle, it is important for optimal asexual development in the blood stages.
与其他真核细胞类型相比，疟原虫似乎拥有更原始的高尔基体，由分散的、未堆叠的顺面和反面高尔基池组成。尽管在寄生虫的分泌途径中起着核心作用，但很少有疟原虫高尔基体驻留蛋白被描述。我们之前鉴定了一种新的未知功能的高尔基体驻留蛋白，我们将其命名为高尔基体蛋白 1，现在表明它与一个以前未被描述的跨膜蛋白（高尔基体蛋白 2，GP2）形成复合物。高尔基蛋白复合物在整个红细胞周期中定位于顺面高尔基，在蚊子阶段也可能存在。分析条件性抑制 GP1 表达和/或失活 GP2 基因的寄生虫株系表明，尽管高尔基体蛋白复合物在寄生虫生命周期的任何阶段都不是必需的，但它对血液阶段的最佳无性发育很重要。
