Centre de recherche en infectiologie, CRCHU de Québec-Université Laval, 2705 Boul. Laurier, Québec, QC G1V 4G2, Canada.
Centre de recherche en infectiologie, CRCHU de Québec-Université Laval, 2705 Boul. Laurier, Québec, QC G1V 4G2, Canada.
Int J Parasitol. 2018 Jan;48(1):13-25. doi: 10.1016/j.ijpara.2017.08.015. Epub 2017 Nov 15.
Despite representing a small percentage of the cellular lipids of eukaryotic cells, phosphoinositides (PIPs) are critical in various processes such as intracellular trafficking and signal transduction. Central to their various functions is the differential distribution of PIP species to specific membrane compartments through the actions of kinases, phosphatases and lipases. Despite their importance in the malaria parasite lifecycle, the subcellular distribution of most PIP species in this organism is still unknown. We here localise several species of PIPs throughout the erythrocytic cycle of Plasmodium falciparum. We show that PI3P is mostly found at the apicoplast and the membrane of the food vacuole, that PI4P associates with the Golgi apparatus and the plasma membrane and that PI(4,5)P2, in addition to being detected at the plasma membrane, labels some cavity-like spherical structures. Finally, we show that the elusive PI5P localises to the plasma membrane, the nucleus and potentially to the transitional endoplasmic reticulum (ER). Our map of the subcellular distribution of PIP species in P. falciparum will be a useful tool to shed light on the dynamics of these lipids in this deadly parasite.
尽管在真核细胞的细胞脂质中只占很小的比例，但磷酸肌醇（PIPs）在各种过程中都至关重要，如细胞内运输和信号转导。它们的各种功能的核心是通过激酶、磷酸酶和脂酶的作用，将 PIP 物种差异分布到特定的膜隔室中。尽管它们在疟原虫生命周期中很重要，但该生物体内大多数 PIP 物种的亚细胞分布仍然未知。我们在这里定位了疟原虫红细胞周期中的几种 PIP 物种。我们表明，PI3P 主要存在于类质体和食物泡的膜上，PI4P 与高尔基体和质膜相关，而 PI(4,5)P2 除了在质膜上被检测到之外，还标记了一些类似腔的球形结构。最后，我们表明，难以捉摸的 PI5P 定位于质膜、核，并可能定位于过渡性内质网（ER）。我们在疟原虫中 PIP 物种亚细胞分布的图谱将是一个有用的工具，可以揭示这些脂质在这种致命寄生虫中的动态。
