Departamento de Química Analítica, Química Física e Ingeniería Química, Instituto de Investigación Química "Andrés M. del Río", Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Departamento de Química Analítica, Química Física e Ingeniería Química, Instituto de Investigación Química "Andrés M. del Río", Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Food Res Int. 2018 Apr;106:458-467. doi: 10.1016/j.foodres.2018.01.015. Epub 2018 Jan 11.
The long exposition to reactive species results in oxidative stress which has been related with the development of cancer and other serious diseases. Olea europaea and Prunus persica seeds present a high protein content and preliminary results demonstrated their high potency to obtain bioactive peptides. The protective effect against oxidative damage exerted by peptides released from Olea europaea and Prunus persica seeds has been evaluated in this work. Seed hydrolysates showed protection against oxidation through four different mechanisms: inhibition of the formation of hydroxyl radicals, scavenging of free radicals, reduction of oxidizing compounds, and inhibition of lipid peroxidation. Moreover, seed hydrolysates also reduced the oxidative stress induced by an oxidizing agent on human cancer cells. Despite protection evaluated by individual mechanisms seemed to be significantly affected by the seed genotype, overall protection of seed hydrolysates was not so different. Seeds hydrolysates were not cytotoxic on normal cells but they demonstrated antiproliferative effect on human cancer cells (HeLa, PC-3, and HT-29). Peptides in all seed hydrolysates were sequenced by RP-HPLC-ESI-Q-TOF. Eighteen common peptides were observed among olive seed hydrolysates while a wider variability was observed among Prunus seed hydrolysates.
长时间暴露在反应性物种中会导致氧化应激，这与癌症和其他严重疾病的发展有关。油橄榄和桃的种子中含有丰富的蛋白质，初步研究结果表明，它们具有产生生物活性肽的巨大潜力。本研究评估了从油橄榄和桃种子中释放的肽对氧化损伤的保护作用。种子水解产物通过四种不同的机制显示出抗氧化作用：抑制羟基自由基的形成、清除自由基、还原氧化化合物和抑制脂质过氧化。此外，种子水解产物还能减轻氧化剂对人癌细胞诱导的氧化应激。尽管个别机制评估的保护作用似乎受到种子基因型的显著影响，但种子水解产物的总体保护作用并没有太大差异。种子水解产物对正常细胞没有细胞毒性，但对人癌细胞（HeLa、PC-3 和 HT-29）具有抗增殖作用。所有种子水解产物中的肽都通过 RP-HPLC-ESI-Q-TOF 进行了测序。在橄榄种子水解产物中观察到 18 种常见肽，而在桃种子水解产物中观察到更广泛的变异性。
