Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization By Shaanxi & Education Ministry, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi University of Chinese Medicine, Xianyang, 712046, China.
School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
Talanta. 2024 Dec 1;280:126702. doi: 10.1016/j.talanta.2024.126702. Epub 2024 Aug 13.
A high performance liquid chromatography-ultraviolet-visible detector-electrospray ionization-ion trap-time-of-flight-mass spectrometry-total antioxidant capacity determination (HPLC-UVD-ESI-IT-TOF-MS-TACD) new online technique was developed for efficient screening of potential antioxidant active components in Prunus persica flowers (PPF) from 4 origins. Through this online system, 46 compounds were initially identified, while 20 compounds with DPPH binding activity and 21 compounds with FRAP binding activity were detected. The antioxidant activities of 9 compounds obtained from the screening were then validated in DNA oxidative damage protection study. The results showed that this online system can cope well with the complexity of the samples. This also provides technical basis for rapid screening of antioxidant resources of PPF. In short, this study made the chemical composition of PPF more abundant and its potential antioxidant active compounds more explicit, which provided new ideas for the detection and development of natural antioxidants and provided scientific basis for PPF as functional food.
建立一种高效液相色谱-紫外-可见检测器-电喷雾离子阱-飞行时间质谱-总抗氧化能力测定（HPLC-UVD-ESI-IT-TOF-MS-TACD）新的在线技术，用于从 4 个产地的桃花（PPF）中有效筛选潜在的抗氧化活性成分。通过该在线系统，初步鉴定出 46 种化合物，同时检测到 20 种具有 DPPH 结合活性的化合物和 21 种具有 FRAP 结合活性的化合物。然后，对筛选得到的 9 种化合物的抗氧化活性进行了 DNA 氧化损伤保护研究验证。结果表明，该在线系统能够很好地应对样品的复杂性。这也为 PPF 抗氧化资源的快速筛选提供了技术基础。总之，本研究使 PPF 的化学成分更加丰富，潜在的抗氧化活性化合物更加明确，为天然抗氧化剂的检测和开发提供了新的思路，为 PPF 作为功能性食品提供了科学依据。
