Kutryk M J, Pierce G N, Dhalla N S
Basic Res Cardiol. 1987 May-Jun;82(3):271-8. doi: 10.1007/BF01906859.
Alterations in the cardiac tissue and serum acid hydrolase activities were studied in chronic streptozotocin-induced diabetes in rats. No changes were observed in total cardiac tissue homogenate lysosomal enzyme activities at 4 weeks of diabetes but there were significant alterations in the distribution of selected enzymes. Significant decreases in nonsedimentable beta-N-acetylglucosaminidase (NAG) and beta-galactosidase (Gal) activities were observed at 4 weeks of diabetes. At 8 weeks of the disease, decreased activities of NAG and Gal were observed in heart homogenates but no changes were apparent in alpha-mannosidase (Man) or acid phosphatase activities. Nonsedimentable activities of NAG and both sedimentable and nonsedimentable activities of Gal were decreased at 8 weeks. At 16 weeks of the diabetic condition, increased activities of NAG, Gal and acid phosphatase were observed. This increase at 16 weeks of the disease was due to an increase in sedimentable enzyme activity. At all times of diabetes, serum enzyme activities were significantly increased. Insulin treatment reversed all of the observed changes in tissue homogenates, but serum levels were not completely reversed. These results suggest that cardiac lysosomal hydrolases are probably only involved in the later stages of the diabetic cardiomyopathy when extensive ultrastructural derangements are evident. The present evidence also suggests that the heart may be a source of serum hydrolase activities.
在链脲佐菌素诱导的大鼠慢性糖尿病模型中，研究了心脏组织和血清酸性水解酶活性的变化。糖尿病4周时，心脏组织匀浆溶酶体酶总活性未见改变，但某些酶的分布有显著变化。糖尿病4周时，不可沉淀的β-N-乙酰氨基葡萄糖苷酶（NAG）和β-半乳糖苷酶（Gal）活性显著降低。病程8周时，心脏匀浆中NAG和Gal活性降低，但α-甘露糖苷酶（Man）或酸性磷酸酶活性无明显变化。8周时，NAG的不可沉淀活性以及Gal的可沉淀和不可沉淀活性均降低。糖尿病16周时，NAG、Gal和酸性磷酸酶活性增加。病程16周时的这种增加是由于可沉淀酶活性增加所致。在糖尿病的各个阶段，血清酶活性均显著升高。胰岛素治疗逆转了组织匀浆中观察到的所有变化，但血清水平未完全逆转。这些结果表明，心脏溶酶体水解酶可能仅在糖尿病性心肌病的后期阶段参与其中，此时广泛的超微结构紊乱明显。目前的证据还表明，心脏可能是血清水解酶活性的来源。
