Acid glycohydrolase in Chinese hamster (Cricetulus griseus) with spontaneous diabetes--V. Subcellular distribution in the kidney.

1. Seven renal glycohydrolases were measured in four subcellular fractions prepared from highly inbred aglycosuric (AV-line) and glycosuric (XA-line) Chinese hamsters. 2. alpha-D-galactosidase and beta-D-galactosidase were highest in the nuclear (N) and supernatant (S) fractions; both fractions showed reduced activities in the XA animals. 3. alpha-D-mannosidase was chiefly a particulate enzyme and its decrease in XA animals was evident in N, lysosomal-mitochondrial (LM) and mitochondrial-microsomal (MM) fractions. 4. No significant difference in N-acetyl-beta-D-glucosaminidase was found in any of these subcellular fractions between AV and XA animals. 5. Although total alpha-L-fucosidase and beta-D-fucosidase levels were similar in AV and XA kidneys, a difference was observed in the S fraction. 6. beta-D-glucuronidase was virtually absent in N and LM fractions and the S fraction of AV kidneys showed higher activity than the XAs.