Wolinsky H, Goldfischer S, Capron L, Capron F, Coltoff-Schiller B, Kasak L
Circ Res. 1978 Jun;42(6):821-31. doi: 10.1161/01.res.42.6.821.
Vascular disease in diabetics could arise in part from altered vessel wall catebolism. Specific activities of hydrolases in aortic smooth muscle cells from rats with streptozotocin-induced diabetes were measured. Enyzmes included: neutral alpha-glucosidase, alpha-mannosidase, and lysosomal N-acetyl beta-glucosaminidase, beta-galactosidase, cathepsin C, acid alpha-glucosidase, and acid cholesteryl esterase. After 4,8, and 11 weeks of diabetes, activities of all enzymes studied were decreased significantly in diabetic vessels, decreases ranging from 15% for cathepsin C to 62% for alpha-mannosidase. After 3 weeks of diabetes, insulin treatment for 1 week restored enzyme levels to normal. After 7 weeks of diabetes, 1 week of insulin treatment did not restore enzyme levels fully to normal (acid cholesteryl esterase was unchanged); 4 weeks of insulin did. Acid phosphatase and N-acetyl beta-glucosaminidase activities were reduced markedly in histochemical studies of diabetic aortas at all time periods and were restored by insulin treatment. Alloxan-induced diabetes gave results similar to those with streptozotocin. Significant decreases of aortic hydrolase activities, including those of lysosomes, occur in experimental diabetes mellitus and could contribute to accumulation of substrates in vascular smooth muscle cells.
糖尿病患者的血管疾病可能部分源于血管壁代谢改变。测定了链脲佐菌素诱导糖尿病大鼠主动脉平滑肌细胞中水解酶的比活性。这些酶包括：中性α-葡萄糖苷酶、α-甘露糖苷酶、溶酶体N-乙酰-β-葡萄糖胺酶、β-半乳糖苷酶、组织蛋白酶C、酸性α-葡萄糖苷酶和酸性胆固醇酯酶。糖尿病4周、8周和11周后，糖尿病血管中所有研究的酶活性均显著降低，降低幅度从组织蛋白酶C的15%到α-甘露糖苷酶的62%不等。糖尿病3周后，胰岛素治疗1周可使酶水平恢复正常。糖尿病7周后，胰岛素治疗1周不能使酶水平完全恢复正常（酸性胆固醇酯酶未改变）；胰岛素治疗4周则可。在糖尿病主动脉的组织化学研究中，酸性磷酸酶和N-乙酰-β-葡萄糖胺酶活性在所有时间段均显著降低，并通过胰岛素治疗得以恢复。四氧嘧啶诱导的糖尿病结果与链脲佐菌素诱导的相似。实验性糖尿病中主动脉水解酶活性显著降低，包括溶酶体酶，这可能导致血管平滑肌细胞中底物的积累。
