López-Hermoso Clara, de la Haba Rafael R, Sánchez-Porro Cristina, Ventosa Antonio
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Int J Syst Evol Microbiol. 2018 May;68(5):1599-1607. doi: 10.1099/ijsem.0.002716. Epub 2018 Mar 26.
We carried out a comparative taxonomic study of Salinivibrio proteolyticus and Salinivibrio costicola subsp. vallismortis, as well as of five halophilic strains (IB574, IB872, PR5, PR919 and PR932), isolated from salterns in Spain and Puerto Rico that were closely related to these bacteria. Multilocus sequence analysis of concatenated gyrB, recA, rpoA and rpoD housekeeping genes showed that they constituted a single cluster separate from the other species and subspecies of Salinivibrio. Experimental and in silico DNA-DNA hybridization studies indicated that they are members of the same species, with relatedness of 100-74 % and 97.8-70.0 %, respectively. The average nucleotide identity (ANI) determined for these strains was 99.7-95.6 % for ANIb and 99.7-95.7 % for OrthoANI. However, the ANI values for S. costicolasubsp.vallismortis DSM 8285 with respect to S. costicolasubsp.costicola DSM 11403 and S. costicolasubsp.alcaliphilus DSM 16359 were 78.7 and 78.9 % (ANIb) and 79.4 and 79.4 % (OrthoANI), respectively. The phylogenomic tree based on 1072 concatenated orthologous single-copy core genes confirmed that S. proteolyticus, S. costicolasubsp.vallismortis and the five new isolates constitute a coherent single phylogroup, separated from the other species and subspecies of Salinivibrio. All these data indicate that S. costicolasubsp.vallismortis is a heterotypic synonym of S. proteolyticus and we propose an emended description of this species.
我们对解蛋白盐弧菌（Salinivibrio proteolyticus）、科氏盐弧菌瓦利斯莫蒂斯亚种（Salinivibrio costicola subsp. vallismortis）以及从西班牙和波多黎各盐场分离出的与这些细菌密切相关的五株嗜盐菌株（IB574、IB872、PR5、PR919和PR932）进行了比较分类学研究。对gyrB、recA、rpoA和rpoD看家基因串联序列的多位点序列分析表明，它们构成了一个与盐弧菌其他种和亚种分开的单一组群。实验性和计算机模拟DNA-DNA杂交研究表明，它们是同一物种的成员，相关性分别为100 - 74%和97.8 - 70.0%。这些菌株的平均核苷酸同一性（ANI），ANIb测定值为99.7 - 95.6%，OrthoANI测定值为99.7 - 95.7%。然而，科氏盐弧菌瓦利斯莫蒂斯亚种DSM 8285相对于科氏盐弧菌科氏亚种DSM 11403和科氏盐弧菌嗜碱亚种DSM 16359的ANI值分别为78.7%和78.9%（ANIb）以及79.4%和79.4%（OrthoANI）。基于1072个串联直系单拷贝核心基因构建的系统发育树证实，解蛋白盐弧菌、科氏盐弧菌瓦利斯莫蒂斯亚种和五株新分离菌株构成一个连贯的单系群，与盐弧菌的其他种和亚种分开。所有这些数据表明，科氏盐弧菌瓦利斯莫蒂斯亚种是解蛋白盐弧菌的异名，我们提议对该物种进行修订描述。
