Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Syst Appl Microbiol. 2018 May;41(3):159-166. doi: 10.1016/j.syapm.2017.12.001. Epub 2017 Dec 21.
Ten Gram-strain-negative, facultatively anaerobic, moderately halophilic bacterial strains, designated AL184, IB560, IB563, IC202, IC317, MA421, ML277, ML318, ML328A and ML331, were isolated from water ponds of five salterns located in Spain. The cells were motile, curved rods and oxidase and catalase positive. All of them grew optimally at 37°C, at pH 7.2-7.4 and in the presence of 7.5% (w/v) NaCl. Based on phylogenetic analyses of the 16S rRNA, the isolates were most closely related to Salinivibrio sharmensis BAG (99.6-98.2% 16S rRNA gene sequence similarity) and Salinivibrio costicola subsp. costicola ATCC 35508 (99.0-98.1%). According to the MLSA analyses based on four (gyrB, recA, rpoA and rpoD) and eight (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA) concatenated gene sequences, the most closely relatives were S. siamensis JCM 14472 (96.8-95.4% and 94.9-94.7%, respectively) and S. sharmensis DSM 18182 (94.0-92.6% and 92.9-92.7%, respectively). In silico DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) showed values of 23.3-44.8% and 80.2-91.8%, respectively with the related species demonstrating that the ten isolates constituted a single novel species of the genus Salinivibrio. Its pangenome and core genome consist of 6041 and 1230 genes, respectively. The phylogeny based on the concatenated orthologous core genes revealed that the ten strains form a coherent phylogroup well separated from the rest of the species of the genus Salinivibrio. The major cellular fatty acids of strain AL184 were C and C. The DNA G+C content range was 51.9-52.5mol% (T) and 50.2-50.9mol% (genome). Based on the phylogenetic-phylogenomic, phenotypic and chemotaxonomic data, the ten isolates represent a novel species of the genus Salinivibrio, for which the name Salinivibrio kushneri sp. nov. is proposed. The type strain is AL184 (=CECT 9177=LMG 29817).
从西班牙五个盐场的水池塘中分离到 10 株革兰氏阴性、兼性厌氧、中度嗜盐的细菌菌株，命名为 AL184、IB560、IB563、IC202、IC317、MA421、ML277、ML318、ML328A 和 ML331。细胞为运动、弯曲的杆状，氧化酶和触酶阳性。所有菌株在 37°C、pH7.2-7.4 和 7.5%（w/v）NaCl 存在下生长最佳。基于 16S rRNA 的系统发育分析，这些分离株与 Salinivibrio sharmensis BAG（99.6-98.2% 16S rRNA 基因序列相似性）和 Salinivibrio costicola subsp. costicola ATCC 35508（99.0-98.1%）最为密切相关。根据基于四个（gyrB、recA、rpoA 和 rpoD）和八个（ftsZ、gapA、gyrB、mreB、pyrH、recA、rpoA 和 topA）串联基因序列的 MLSA 分析，最密切相关的是 S. siamensis JCM 14472（96.8-95.4% 和 94.9-94.7%）和 S. sharmensis DSM 18182（94.0-92.6% 和 92.9-92.7%）。在 DNA-DNA 杂交（GGDC）和平均核苷酸同一性（ANI）的计算中，与相关种的数值分别为 23.3-44.8%和 80.2-91.8%，这表明这 10 株菌构成了一个新的盐单胞菌属物种。其泛基因组和核心基因组分别由 6041 和 1230 个基因组成。基于串联直系同源核心基因的系统发育分析表明，这 10 株菌形成了一个与盐单胞菌属的其他物种分离良好的连贯的系统发育群。菌株 AL184 的主要细胞脂肪酸为 C 和 C。DNA G+C 含量范围为 51.9-52.5mol%（T）和 50.2-50.9mol%（基因组）。基于系统发育-系统基因组学、表型和化学分类学数据，这 10 株菌代表了盐单胞菌属的一个新种，命名为 Salinivibrio kushneri sp. nov.。模式菌株为 AL184（=CECT 9177=LMG 29817）。
