Galisteo Cristina, Sánchez-Porro Cristina, de la Haba Rafael R, López-Hermoso Clara, Fernández Ana B, Farias María E, Ventosa Antonio
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas, Planta Piloto de Procesos Industriales Microbiológicos, Centro Científico Tecnológico, CONICET Tucumán T4000, Argentina.
Microorganisms. 2019 Aug 5;7(8):241. doi: 10.3390/microorganisms7080241.
The genus belongs to the family and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35, was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35, including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and DNA-DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%-20% (w/v) NaCl, pH 6-10, and 10-42 °C, with optimum growth at 7.0%-7.5% (w/v) NaCl, pH 8.0, and 37 °C, respectively. Strain S35 was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C, C 7 and C 6, and C 7 and/or C 6. ANI, GGDC, and core-genome analysis determined that strain S35 constitutes a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S35 (= CECT 9634 = BNM 0535).
该属属于 科，包括革兰氏染色阴性、通过极生鞭毛运动且兼性厌氧的弯曲杆菌。它们是嗜盐细菌，常见于高盐水生栖息地和腌制食品中。该属包括五个种和两个亚种。一株推测的新物种菌株S35，先前从高海拔火山、碱性和盐湖索孔帕（阿根廷安第斯山脉）分离得到。在本研究中，我们对菌株S35进行了完整的分类学特征分析，包括16S rRNA基因序列和核心基因组分析、平均核苷酸同一性（ANIb、ANIm和orthoANI）以及DNA-DNA杂交（GGDC），还有表型和化学分类学特征分析。它在3%-20%（w/v）NaCl、pH 6-10和10-42°C下生长，分别在7.0%-7.5%（w/v）NaCl、pH 8.0和37°C下生长最佳。菌株S35氧化酶和过氧化氢酶呈阳性，能够从D-葡萄糖和其他碳水化合物产酸。DNA水解、甲基红试验以及硝酸盐和亚硝酸盐还原试验均为阳性。其主要脂肪酸为C、C 7和C 6，以及C 7和/或C 6。ANI、GGDC和核心基因组分析确定菌株S35构成该属的一个新物种，为此提议命名为 新种。模式菌株为S35（=CECT 9634 = BNM 0535）。
