Laboratory for Cancer Biology, Biomedicine, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Denmark.
Laboratory for Medical Mass Spectrometry, Biomedicine, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Denmark; Clinical Cancer Research Center, Aalborg University Hospital, Aalborg, Denmark.
J Proteomics. 2019 Jan 6;190:27-34. doi: 10.1016/j.jprot.2018.03.013. Epub 2018 Mar 23.
Satellite glial cells (SGCs) in sensory ganglia contribute to the pathogenesis of chronic pain, potentially through mediating extracellular or paracrine signaling. Recently, extracellular vesicles (EVs) in the form of exosomes have been found to play an important role in cell-cell communication. However, their release from SGCs and extent in modulating pain remain unknown. An in vitro cell platform using fresh primary SGCs was used to characterize the shed vesicles by size and proteomic profiling following activation of SGCs by lipopolysaccharide (LPS), simulating neurogenic inflammation in vivo. Results demonstrated that SGCs shed vesicles in the size range of exosomes (>150 nm) but with altered protein expression upon LPS-activation. Proteomic profiling of SGCs-shed EVs showed that a number of proteins were differentially regulated upon LPS stimulation such as junction plakoglobin and myosin 9 that are proposed as novel biomarkers of SGCs activation under inflammatory conditions. Findings from this study highlight the utility of using fresh primary SGC cultures as a model to further investigate EVs under normal and inflammatory conditions. SIGNIFICANCE: This study demonstrated that.
卫星胶质细胞（SGCs）在感觉神经节中有助于慢性疼痛的发病机制，可能通过介导细胞外或旁分泌信号。最近，以外泌体形式存在的细胞外囊泡（EVs）在细胞间通讯中发挥着重要作用。然而，它们从 SGCs 中的释放及其在调节疼痛中的作用尚不清楚。使用新鲜的原代 SGCs 的体外细胞平台，通过用脂多糖（LPS）激活 SGCs 来模拟体内神经原性炎症，来对 SGCs 释放的囊泡进行大小和蛋白质组学分析。结果表明，SGCs 释放的囊泡大小在 exosomes（>150nm）范围内，但在 LPS 激活后蛋白表达发生改变。对 SGCs 释放的 EVs 的蛋白质组学分析表明，许多蛋白质在 LPS 刺激下差异调节，如连接斑蛋白和肌球蛋白 9，它们被认为是炎症条件下 SGCs 激活的新型生物标志物。这项研究的结果强调了使用新鲜的原代 SGC 培养物作为模型在正常和炎症条件下进一步研究 EVs 的效用。意义：本研究表明。
