Taylor-Robinson D, Tuffrey M
Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex.
J Med Microbiol. 1987 Sep;24(2):169-73. doi: 10.1099/00222615-24-2-169.
Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obtained by these methods on vaginal specimens taken on 4 or 5 occasions during 41-42 days were compared with those obtained in cell culture and to a less extent by the MicroTrak direct immunofluorescence test. In comparison with culture, the Chlamydiazyme assay had a sensitivity of 62% and a specificity of 92%; IDEIA had a sensitivity of 76% and a specificity of 94%. These assays sometimes did not detect chlamydiae in specimens taken immediately before specimens which proved positive by culture and the immunoassays were less sensitive if swabs were taken after those for culture. The IDEIA also failed to detect chlamydiae in the late stage of the murine infection when chlamydial elementary bodies were seen by immunofluorescence. The implications of the observations for investigations in the human field as well as for further studies in the mouse are discussed.
在衣原体生殖道感染的小鼠模型中对两种衣原体酶免疫测定法，即衣原体酶测定法（Chlamydiazyme）和免疫诊断酶免疫测定法（IDEIA）进行了评估。分别对10只和11只小鼠的标本进行了衣原体酶测定法和免疫诊断酶免疫测定法评估。这些动物感染了沙眼衣原体SA2f菌株，将这两种方法在41至42天内4或5次采集的阴道标本上获得的结果，与细胞培养获得的结果以及在较小程度上与MicroTrak直接免疫荧光试验获得的结果进行了比较。与培养法相比，衣原体酶测定法的灵敏度为62%，特异性为92%；免疫诊断酶免疫测定法的灵敏度为76%，特异性为94%。这些测定法有时无法在培养和免疫测定法均证明为阳性的标本之前立即采集的标本中检测到衣原体，如果在用于培养的拭子之后采集拭子，免疫测定法的灵敏度会更低。在小鼠感染后期，当通过免疫荧光观察到衣原体原体时，免疫诊断酶免疫测定法也未能检测到衣原体。讨论了这些观察结果对人类领域研究以及小鼠进一步研究的意义。
