a Stem Cell Institute , Kangwon National University , Chuncheon , Gangwon , Republic of Korea.
b College of Veterinary Medicine , Kangwon National University , Chuncheon , Gangwon , Republic of Korea.
Artif Cells Nanomed Biotechnol. 2018;46(sup2):273-284. doi: 10.1080/21691401.2018.1457037. Epub 2018 Mar 27.
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that AgNPs improved the ultra-structure of DLs' collagen fibres with good porosity and increased DLs' resistance against in vitro degradation with good cytocompatibility. AgNPs decreased the host inflammatory reaction against implanted porcine DL slices in vivo and increased the polarization of M2 macrophages. Thus, structural and functional improvements of Porcine DLs could be achieved using AgNPs.
目前尚未发现理想的用于去细胞肝脏（DL）的交联剂。在这项研究中，我们评估了用银纳米粒子（AgNPs）交联后猪 DL 的结构改善和生物相容性。猪肝切片在优化的最高无毒浓度（5μg/mL）下用人肝癌（HepG2）和 EAhy926 人内皮细胞系负载 AgNPs（100nm）后进行去细胞化，然后用 AgNPs 进行交联，并用戊二醛、碳化二亚胺盐酸盐和 N-羟基琥珀酰亚胺进行交联效果比较。结果表明，AgNPs 改善了 DL 胶原纤维的超微结构，具有良好的多孔性，并提高了 DL 抵抗体外降解的能力，具有良好的细胞相容性。AgNPs 减少了宿主对植入的猪 DL 切片的炎症反应，并增加了 M2 巨噬细胞的极化。因此，AgNPs 可以实现猪 DL 的结构和功能改善。
