Agsteribbe E, Hartog M
Laboratory of Physiological Chemistry, State University Groningen, The Netherlands.
Nucleic Acids Res. 1987 Sep 25;15(18):7249-63. doi: 10.1093/nar/15.18.7249.
Neurospora mitochondrial DNA is transcribed into long molecules containing the information of several genes. Processing leads to formation of functionally active RNAs. It has been shown previously that when tRNA sequences are present in these transcripts excision of mRNAs occurs at the acceptor stem of these tRNA sequences. We have investigated the processing of precursor RNAs transcribed from a region of the mitochondrial genome devoid of tRNA genes. This region comprises the genes encoding subunit 6 of the mitochondrial ATPase, subunit 2 of cytochrome aa3 and a mitochondrial ATPase proteolipid-like gene. We have proved that a common precursor of the putative mRNAs of these genes exists and we have determined the positions of the 5' and 3' ends of processing intermediates and of the mature mRNAs. We will discuss possible processing routes and secondary structures that substitute for tRNA sequences as processing sites.
粗糙脉孢菌线粒体DNA被转录成长分子，这些长分子包含几个基因的信息。加工过程导致形成功能活性RNA。先前已经表明，当这些转录本中存在tRNA序列时，mRNA的切除发生在这些tRNA序列的受体茎处。我们研究了从线粒体基因组中一个不含tRNA基因的区域转录的前体RNA的加工过程。该区域包括编码线粒体ATP酶亚基6、细胞色素aa3亚基2和一个线粒体ATP酶蛋白脂质样基因的基因。我们已经证明这些基因的假定mRNA存在一个共同前体，并且我们已经确定了加工中间体和成熟mRNA的5'和3'末端的位置。我们将讨论可能的加工途径以及替代tRNA序列作为加工位点的二级结构。
