Macino G, Morelli G
J Biol Chem. 1983 Nov 10;258(21):13230-5.
The nucleotide sequence of the cytochrome oxidase subunit 2 (COX2) gene has been obtained from cloned mitochondrial DNA segments of Neurospora crassa. The coding sequences have been identified on the basis of protein sequence homology with the subunit 2 of cytochrome oxidase from yeast and man. The postulated precursor of the N. crassa subunit 2 protein is 250 amino acids long, with a molecular weight of 28,700. As in the tRNA and rRNA genes, the subunit 2 gene is flanked by G + C-rich palindromic sequences, which are highly conserved in N. crassa mitochondria. Three major transcripts have been detected by Northern blot hybridization. A transcript of 1100 bases is tentatively considered the fully processed mRNA. Furthermore, S1 nuclease protection experiments have revealed that the putative subunit 2 mRNA has a 330 nucleotide long 5' leader sequence.
已经从粗糙脉孢菌克隆的线粒体DNA片段中获得了细胞色素氧化酶亚基2（COX2）基因的核苷酸序列。根据与酵母和人类细胞色素氧化酶亚基2的蛋白质序列同源性确定了编码序列。推测的粗糙脉孢菌亚基2蛋白前体长度为250个氨基酸，分子量为28,700。与tRNA和rRNA基因一样，亚基2基因两侧是富含G + C的回文序列，这些序列在粗糙脉孢菌线粒体中高度保守。通过Northern印迹杂交检测到三种主要转录本。一个1100个碱基的转录本被初步认为是完全加工的mRNA。此外，S1核酸酶保护实验表明，推定的亚基2 mRNA具有330个核苷酸长的5'前导序列。
