Wild J, Filutowicz M, Kłopotowski T
Arch Microbiol. 1978 Jul;118(1):71-7. doi: 10.1007/BF00406077.
Utilization of D-amino acids being substrates of D-amino acid dehydrogenase of Salmonella typhimurium was examined. The experiments were done with wild type strains and the mutants dadA missing the enzyme activity and dadR in which its synthesis is released from catabolite repression. Growth on D-tryptophan, D-histidine and D-methionine used as precursors of the L-amino acids was faster when the respective auxotrophs carried dadR mutations. The dadR mutants grew faster when D-or L-alanine was present as a sole source of nitrogen. Experiments with D-amino acid dehydrogenase in vitro provided evidence that D-tryptophan is its substrate with a very low affinity to the dehydrogenase.
研究了鼠伤寒沙门氏菌D -氨基酸脱氢酶底物D -氨基酸的利用情况。实验使用野生型菌株以及缺乏该酶活性的dadA突变体和其合成不受分解代谢物阻遏的dadR突变体进行。当相应的营养缺陷型菌株携带dadR突变时，以D -色氨酸、D -组氨酸和D -甲硫氨酸作为L -氨基酸前体的生长速度更快。当D -或L -丙氨酸作为唯一氮源时，dadR突变体生长得更快。体外D -氨基酸脱氢酶实验提供了证据，表明D -色氨酸是其底物，但对脱氢酶的亲和力非常低。
