鼠伤寒沙门氏菌中D-组氨酸的利用受亮氨酸应答调节蛋白（Lrp）的控制。
D-histidine utilization in Salmonella typhimurium is controlled by the leucine-responsive regulatory protein (Lrp).
作者信息
Hecht K, Zhang S, Klopotowski T, Ames G F
机构信息
Department of Molecular and Cell Biology, University of California at Berkeley 94720, USA.
出版信息
J Bacteriol. 1996 Jan;178(2):327-31. doi: 10.1128/jb.178.2.327-331.1996.
Abstract
A new class of D-histidine-utilizing mutants which carry mutations in the gene encoding the leucine-responsive regulatory protein (Lrp) has been identified in Salmonella typhimurium. The lrp mutations arise as suppressors of mutations in the genes encoding the histidine permease which drastically decrease the level of histidine transport activity. However, the suppressor effect is not exerted by elevating the level of the permease. Rather, the properties of the suppressor mutants are consistent with the notion that the parent permease mutants transport D-histidine at a low level and that in the suppressor mutants D-histidine is utilized effectively through elevated levels of racemization. The enzymatic activity of D-alanine dehydrogenase (Dad) is shown to be elevated in the suppressor mutants and is a possible pathway of D-histidine utilization. The suppressor mutations are located in the helix-turn-helix region of Lrp.
摘要
在鼠伤寒沙门氏菌中已鉴定出一类利用D-组氨酸的新突变体，这些突变体在编码亮氨酸响应调节蛋白（Lrp）的基因中携带突变。lrp突变作为编码组氨酸通透酶的基因突变的抑制子出现，这些突变会大幅降低组氨酸转运活性水平。然而，抑制作用并非通过提高通透酶水平来实现。相反，抑制突变体的特性与以下观点一致：亲本通透酶突变体以低水平转运D-组氨酸，而在抑制突变体中，通过提高消旋化水平可有效利用D-组氨酸。D-丙氨酸脱氢酶（Dad）的酶活性在抑制突变体中升高，这是D-组氨酸利用的一条可能途径。抑制突变位于Lrp的螺旋-转角-螺旋区域。
相似文献
1
D-histidine utilization in Salmonella typhimurium is controlled by the leucine-responsive regulatory protein (Lrp).鼠伤寒沙门氏菌中D-组氨酸的利用受亮氨酸应答调节蛋白（Lrp）的控制。
J Bacteriol. 1996 Jan;178(2):327-31. doi: 10.1128/jb.178.2.327-331.1996.
2
Salmonella typhimurium histidine periplasmic permease mutations that allow transport in the absence of histidine-binding proteins.鼠伤寒沙门氏菌组氨酸周质通透酶突变，其在缺乏组氨酸结合蛋白的情况下仍能进行转运。
J Bacteriol. 1991 Feb;173(4):1444-51. doi: 10.1128/jb.173.4.1444-1451.1991.
3
Reconstitution of a bacterial periplasmic permease in proteoliposomes and demonstration of ATP hydrolysis concomitant with transport.细菌周质通透酶在蛋白脂质体中的重构及伴随转运的ATP水解的证明。
Proc Natl Acad Sci U S A. 1989 Sep;86(18):6953-7. doi: 10.1073/pnas.86.18.6953.
4
A chimeric nucleotide-binding protein, encoded by a hisP-malK hybrid gene, is functional in maltose transport in Salmonella typhimurium.一种由hisP-malK杂交基因编码的嵌合核苷酸结合蛋白在鼠伤寒沙门氏菌的麦芽糖转运中起作用。
Mol Microbiol. 1991 Jun;5(6):1375-83. doi: 10.1111/j.1365-2958.1991.tb00784.x.
5
Overproduction of the membrane-bound components of the histidine permease from Salmonella typhimurium: identification of the M protein.鼠伤寒沙门氏菌组氨酸通透酶膜结合成分的过量产生：M蛋白的鉴定。
Biochimie. 1985 Jan;67(1):149-54. doi: 10.1016/s0300-9084(85)80242-x.
6
Sequence of the complete P protein gene and part of the M protein gene from the histidine transport operon of Escherichia coli compared to that of Salmonella typhimurium.将大肠杆菌组氨酸转运操纵子的完整P蛋白基因序列和部分M蛋白基因序列与鼠伤寒沙门氏菌的进行比较。
Nucleic Acids Res. 1987 Oct 26;15(20):8568. doi: 10.1093/nar/15.20.8568.
7
Binding protein-independent histidine permease mutants. Uncoupling of ATP hydrolysis from transmembrane signaling.不依赖结合蛋白的组氨酸通透酶突变体。ATP水解与跨膜信号转导的解偶联。
J Biol Chem. 1991 Sep 5;266(25):16293-6.
8
Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine.影响大肠杆菌Lrp结合DNA、激活转录或对亮氨酸作出反应能力的突变。
J Bacteriol. 1993 Feb;175(4):1110-7. doi: 10.1128/jb.175.4.1110-1117.1993.
9
Autoregulated expression of the gene coding for the leucine-responsive protein, Lrp, a global regulator in Salmonella enterica serovar Typhimurium.编码亮氨酸应答蛋白Lrp（鼠伤寒沙门氏菌中的一种全局调节因子）的基因的自动调节表达。
Microbiology (Reading). 2008 Jul;154(Pt 7):2008-2016. doi: 10.1099/mic.0.2008/018358-0.
10
Utilization of D-amino acids by dadR mutants of Salmonella typhimurium.鼠伤寒沙门氏菌dadR突变体对D-氨基酸的利用
Arch Microbiol. 1978 Jul;118(1):71-7. doi: 10.1007/BF00406077.
引用本文的文献
1
Evidence from Mutational Analysis for a Single Transmembrane Substrate Binding Site in the Histidine ATP-Binding Cassette Transporter of Salmonella enterica Serovar Typhimurium.从突变分析中得到的沙门氏菌组氨酸 ATP 结合盒转运蛋白的单一跨膜底物结合位点的证据。
J Bacteriol. 2018 Dec 20;201(2). doi: 10.1128/JB.00521-18. Print 2019 Jan 15.
2
The Bacterial Two-Hybrid System Uncovers the Involvement of Acetylation in Regulating of Lrp Activity in Typhimurium.细菌双杂交系统揭示了乙酰化在鼠伤寒沙门氏菌中对Lrp活性调控的参与。
Front Microbiol. 2016 Nov 17;7:1864. doi: 10.3389/fmicb.2016.01864. eCollection 2016.
3
Unexpected coregulator range for the global regulator Lrp of Escherichia coli and Proteus mirabilis.出乎意料的全局调控因子 Lrp 对大肠杆菌和奇异变形杆菌的共同调控范围。
J Bacteriol. 2011 Mar;193(5):1054-64. doi: 10.1128/JB.01183-10. Epub 2010 Dec 17.
4
Leucine-responsive regulatory protein (Lrp) acts as a virulence repressor in Salmonella enterica serovar Typhimurium.亮氨酸响应调节蛋白（Lrp）在鼠伤寒沙门氏菌中作为一种毒力抑制因子发挥作用。
J Bacteriol. 2009 Feb;191(4):1278-92. doi: 10.1128/JB.01142-08. Epub 2008 Dec 12.
5
Comparison of DeltarelA strains of Escherichia coli and Salmonella enterica serovar Typhimurium suggests a role for ppGpp in attenuation regulation of branched-chain amino acid biosynthesis.大肠杆菌和鼠伤寒沙门氏菌DeltarelA菌株的比较表明，ppGpp在支链氨基酸生物合成的衰减调节中起作用。
J Bacteriol. 2001 Nov;183(21):6184-96. doi: 10.1128/JB.183.21.6184-6196.2001.
6
Two roles for the leucine-responsive regulatory protein in expression of the alanine catabolic operon (dadAB) in Klebsiella aerogenes.亮氨酸应答调节蛋白在产气克雷伯菌丙氨酸分解代谢操纵子（dadAB）表达中的两种作用。
J Bacteriol. 1999 Feb;181(3):1054-8. doi: 10.1128/JB.181.3.1054-1058.1999.
7
Alanine catabolism in Klebsiella aerogenes: molecular characterization of the dadAB operon and its regulation by the nitrogen assimilation control protein.产气克雷伯菌中的丙氨酸分解代谢：dadAB操纵子的分子特征及其受氮同化控制蛋白的调控
J Bacteriol. 1998 Feb;180(3):563-70. doi: 10.1128/JB.180.3.563-570.1998.
8
Use of an inducible regulatory protein to identify members of a regulon: application to the regulon controlled by the leucine-responsive regulatory protein (Lrp) in Escherichia coli.利用可诱导调节蛋白鉴定调节子成员：应用于大肠杆菌中由亮氨酸应答调节蛋白（Lrp）控制的调节子
J Bacteriol. 1997 Oct;179(20):6254-63. doi: 10.1128/jb.179.20.6254-6263.1997.
9
Lrp is a direct repressor of the dad operon in Escherichia coli.Lrp是大肠杆菌中dad操纵子的直接阻遏物。
J Bacteriol. 1996 Dec;178(24):7234-40. doi: 10.1128/jb.178.24.7234-7240.1996.
本文引用的文献
1
UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM.鼠伤寒沙门氏菌对氨基酸的摄取
Arch Biochem Biophys. 1964 Jan;104:1-18. doi: 10.1016/s0003-9861(64)80028-x.
2
Leucine-responsive regulatory protein: a global regulator of gene expression in E. coli.亮氨酸应答调节蛋白：大肠杆菌基因表达的全局调节因子。
Annu Rev Microbiol. 1995;49:747-75. doi: 10.1146/annurev.mi.49.100195.003531.
3
Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine.影响大肠杆菌Lrp结合DNA、激活转录或对亮氨酸作出反应能力的突变。
J Bacteriol. 1993 Feb;175(4):1110-7. doi: 10.1128/jb.175.4.1110-1117.1993.
4
The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli.亮氨酸响应调节蛋白，大肠杆菌新陈代谢的全局调节因子。
Microbiol Rev. 1994 Sep;58(3):466-90. doi: 10.1128/mr.58.3.466-490.1994.
5
The amino acid sequence of Lrp is highly conserved in four enteric microorganisms.Lrp的氨基酸序列在四种肠道微生物中高度保守。
J Bacteriol. 1995 Mar;177(6):1624-6. doi: 10.1128/jb.177.6.1624-1626.1995.
6
Structure/function analysis of the periplasmic histidine-binding protein. Mutations decreasing ligand binding alter the properties of the conformational change and of the closed form.周质组氨酸结合蛋白的结构/功能分析。降低配体结合能力的突变会改变构象变化及封闭形式的特性。
J Biol Chem. 1995 Jul 7;270(27):16097-106. doi: 10.1074/jbc.270.27.16097.
7
8
Two alanine racemase genes in Salmonella typhimurium that differ in structure and function.鼠伤寒沙门氏菌中两个结构和功能不同的丙氨酸消旋酶基因。
J Bacteriol. 1983 Mar;153(3):1439-50. doi: 10.1128/jb.153.3.1439-1450.1983.
9
D-Amino acid dehydrogenase of Escherichia coli K12: positive selection of mutants defective in enzyme activity and localization of the structural gene.大肠杆菌K12的D-氨基酸脱氢酶：酶活性缺陷型突变体的正向选择及结构基因的定位
Mol Gen Genet. 1981;181(3):373-8. doi: 10.1007/BF00425614.
10
Analysis of promoter mutations in the histidine transport operon of Salmonella typhimurium: use of hybrid M13 bacteriophages for cloning, transformation, and sequencing.鼠伤寒沙门氏菌组氨酸转运操纵子启动子突变分析：利用杂交M13噬菌体进行克隆、转化及测序
J Bacteriol. 1984 Sep;159(3):1000-5. doi: 10.1128/jb.159.3.1000-1005.1984.
Zotero 插件