牛肾上腺嗜铬颗粒是心钠素的合成部位。

Bovine adrenal chromaffin granules are a site of synthesis of atrial natriuretic factor.

作者信息

Ong H, Lazure C, Nguyen T T, McNicoll N, Seidah N, Chrétien M, De Léan A

机构信息

Clinical Research Institute of Montreal, Quebec, Canada.

出版信息

Biochem Biophys Res Commun. 1987 Sep 30;147(3):957-63. doi: 10.1016/s0006-291x(87)80163-8.

DOI:10.1016/s0006-291x(87)80163-8

PMID:2959287

Abstract

We have previously reported the existence of a peptide factor in the adrenal medulla which inhibits aldosterone secretion in cultured bovine zona glomerulosa cells. The acid extracts of chromaffin granules from bovine adrenal medulla were purified by a four step high performance liquid chromatography procedure. Two active fractions exhibited sequence homology with bovine atrial natriuretic factor ANF (Ser99-Tyr126) and its polypeptide precursor (Asn1-Tyr126). The occurrence of both precursor and mature forms of ANF within chromaffin granules indicates the endogenous character of ANF in the adrenal medulla and suggests the potential usefulness of cultured adrenal chromaffin cells for investigating the synthesis, maturation and secretion of atrial peptides.

摘要

我们之前曾报道，肾上腺髓质中存在一种肽因子，它可抑制培养的牛肾小球带细胞中的醛固酮分泌。牛肾上腺髓质嗜铬颗粒的酸提取物通过四步高效液相色谱法进行纯化。两个活性级分与牛心钠素ANF（Ser99-Tyr126）及其多肽前体（Asn1-Tyr126）表现出序列同源性。嗜铬颗粒中同时存在ANF的前体和成熟形式，这表明肾上腺髓质中ANF具有内源性特征，并提示培养的肾上腺嗜铬细胞在研究心房肽的合成、成熟和分泌方面可能具有实用性。