Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte, Natal, Brazil.
Laboratório de Biologia Molecular e Genômica, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
Sci Rep. 2018 Mar 28;8(1):5327. doi: 10.1038/s41598-018-23708-5.
Chromobacterium violaceum is a ß-proteobacterium found widely worldwide with important biotechnological properties and is associated to lethal sepsis in immune-depressed individuals. In this work, we report the discover, complete sequence and annotation of a plasmid detected in C. violaceum that has been unnoticed until now. We used DNA single-molecule analysis to confirm that the episome found was a circular molecule and then proceeded with NGS sequencing. After DNA annotation, we found that this extra-chromosomal DNA is probably a defective bacteriophage of approximately 44 kilobases, with 39 ORFs comprising, mostly hypothetical proteins. We also found DNA sequences that ensure proper plasmid replication and partitioning as well as a toxin addiction system. This report sheds light on the biology of this important species, helping us to understand the mechanisms by which C. violaceum endures to several harsh conditions. This discovery could also be a first step in the development of a DNA manipulation tool in this bacterium.
类鼻疽伯克霍尔德菌是一种广泛分布于世界各地的β-变形菌，具有重要的生物技术特性，与免疫功能低下个体的致死性败血症有关。在这项工作中，我们报告了一种在类鼻疽伯克霍尔德菌中发现的、以前未被注意到的质粒的发现、完整序列和注释。我们使用 DNA 单分子分析来确认发现的附加体是一个圆形分子，然后进行 NGS 测序。在 DNA 注释之后，我们发现这个染色体外 DNA 可能是一个大约 44 千碱基对的缺陷噬菌体，包含 39 个 ORF，主要是假设蛋白。我们还发现了确保质粒正确复制和分配的 DNA 序列，以及一种毒素成瘾系统。本报告揭示了这一重要物种的生物学特性，帮助我们了解类鼻疽伯克霍尔德菌如何耐受多种恶劣条件的机制。这一发现也可能是在该细菌中开发 DNA 操作工具的第一步。
