Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200127, China.
Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Institutes of Biomedical Sciences and Institute of Medical Microbiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Emerg Microbes Infect. 2018 Mar 29;7(1):45. doi: 10.1038/s41426-018-0048-7.
Staphylococcus aureus (S. aureus) is an important pathogen of humans and livestock species, but an understanding of the clonal distribution of S. aureus causing different host-species infections in the same geographical environment and within the same period is lacking. By characterizing infections caused by S. aureus in bovine, pediatric, and adult patients in Shanghai, China, between 2012 and 2014, we identified methicillin-sensitive S. aureus (MSSA) ST188 as the major lineage causing infections in multiple host species. Whole-genome sequencing and phenotypic analyses demonstrated that ST188 might evolve from livestock, and there was no significant genomic or virulence difference between ST188 isolated from livestock and humans. The virulence of ST188 is related to its adhesion and nasal colonization ability. This result is in accord with the strong epithelial cell adhesion and biofilm formation properties of ST188. Furthermore, the adhesion- and biofilm-formation-related genes are present in multiple copies and exhibit significantly increased expression in ST188. In conclusion, S. aureus ST188 is the major lineage causing human and livestock infections in Shanghai, China. Due to its high expression of the factors associated with bacterial adhesion and biofilm formation, ST188 has the ability to colonize and infect different host species.
金黄色葡萄球菌（S. aureus）是人类和家畜物种的重要病原体，但对于在同一地理环境和同一时期内引起不同宿主物种感染的金黄色葡萄球菌的克隆分布情况，我们还缺乏了解。通过对 2012 年至 2014 年期间在中国上海的牛、儿科和成人患者的金黄色葡萄球菌感染进行特征描述，我们发现耐甲氧西林金黄色葡萄球菌（MSSA）ST188 是引起多种宿主物种感染的主要谱系。全基因组测序和表型分析表明，ST188 可能起源于家畜，并且从家畜和人类中分离出的 ST188 在基因组或毒力方面没有显著差异。ST188 的毒力与其黏附能力和鼻腔定植能力有关。这一结果与 ST188 具有较强的上皮细胞黏附和生物膜形成特性相符。此外，与黏附和生物膜形成相关的基因以多个拷贝存在，并在 ST188 中呈现出显著增加的表达。总之，金黄色葡萄球菌 ST188 是引起中国上海人类和家畜感染的主要谱系。由于其与细菌黏附及生物膜形成相关的因子表达水平较高，ST188 具有定植和感染不同宿主物种的能力。
