College of Pharmacy, and Chongqing Pharmacodynamic Evaluation Engineering Technology Research Center, Chongqing Medical University, Box 380#, 1 Yi Xue Yuan Road, Chongqing, 400016, People's Republic of China.
Mikrochim Acta. 2017 Dec 18;185(1):55. doi: 10.1007/s00604-017-2563-y.
This research describes a nanowire network-based method for detecting the activity of myeloperoxidase (MPO), a biomarker of acute coronary syndromes (ACS). Trimetallic CuPdPt nanowire networks (CuPdPt NWNWs) were prepared by a one-step chemical reduction method. The metallic precursors quickly form nanowire network structures without the need for additional capping agents or surfactants. This process creates a product with a clean surface. The NWNWs were dropped onto a glassy carbon electrode (GCE) to obtain a sensor with good catalytic activity towards the reduction of hydrogen peroxide (HO), which was used as an electrochemical probe working at -0.4 V (vs. SCE). It also provided a large surface for further modification. Next, an antibody against MPO was immobilized on the modified GCE via the stable conjunction between Cu, Pt, Pd and amino groups. Upon binding of MPO to the antibody on the GCE, the current response to HO was reduced by 35 μA·cm. The immunosensor had a linear response within the 100 fg·mL to 50 ng·mL MPO concentration range and a 33 fg·mL detection limit (at an S/N ratio of 3). The recovery of spiked serum samples ranged from 99.8 to 103.6%. This result suggests that the method can be applied to the quantitation of MPO in human serum samples. Graphical abstract A trimetallic CuPdPt nanowire networks was placed on a glassy cabon electrode (GCE) to design an immunosensor for myeloperoxidase (MPO), a biomarker for the acute coronary syndrome (ACS). Antibody against MPO was immobilized on the network via conjugation between Cu, Pt, Pd and amino groups. Amperometric i-t measurements were conducted to quantify the amount of MPO that binds to the antibody on the surface of the modified GCE.
本研究描述了一种基于纳米线网络的方法，用于检测髓过氧化物酶（MPO）的活性，MPO 是急性冠状动脉综合征（ACS）的生物标志物。通过一步化学还原法制备了三元 CuPdPt 纳米线网络（CuPdPt NWNWs）。金属前体迅速形成纳米线网络结构，无需额外的封端剂或表面活性剂。这一过程创造了一种表面清洁的产物。将 NWNWs 滴到玻碳电极（GCE）上，获得了对过氧化氢（HO）还原具有良好催化活性的传感器，HO 用作在 -0.4 V（相对于 SCE）下工作的电化学探针。它还提供了一个用于进一步修饰的大表面。接下来，通过 Cu、Pt、Pd 与氨基之间的稳定结合，将针对 MPO 的抗体固定在修饰的 GCE 上。当 MPO 与 GCE 上的抗体结合时，HO 的电流响应降低了 35 μA·cm。免疫传感器在 100 fg·mL 至 50 ng·mL MPO 浓度范围内具有线性响应，检测限为 33 fg·mL（信噪比为 3）。加标血清样品的回收率范围为 99.8%至 103.6%。结果表明，该方法可应用于人血清样品中 MPO 的定量。
