Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2013 Aug 15;46:91-6. doi: 10.1016/j.bios.2013.02.014. Epub 2013 Feb 26.
Dumbbell-like Pt-Fe₃O₄ nanoparticles (NPs) were synthesized and used as a novel kind of label for the preparation of electrochemical immunosensor, which is applied to the detection of cancer biomarker squamous cell carcinoma antigen (SCC-Ag). The signal amplification strategy, using the synergetic effect present in Pt-Fe₃O₄ to increase the reduction ability of the NPs toward H₂O₂, improved the sensitivity of the immunosensor. Nitrogen-doped graphene sheets (N-GS) were synthesized from graphite oxides through thermal annealing of graphite oxides in ammonia, which was used to immobilize primary anti-SCC antibody (Ab₁). Secondary anti-SCC antibody (Ab₂) was adsorbed onto the Pt-Fe₃O₄ NPs. The immunosensor was prepared through a sandwich structure and displayed a wide linear range (0.05-18 ng/mL), low detection limit (15.3 pg/mL), good reproducibility and stability. The method has been applied to the analysis of clinical serum samples with satisfactory results. These labels for immunosensors can provide many potential applications for the detection of different biomolecules.
哑铃状的 Pt-Fe₃O₄ 纳米粒子 (NPs) 被合成并用作电化学免疫传感器制备的新型标记物，用于检测癌症生物标志物鳞状细胞癌抗原 (SCC-Ag)。信号放大策略利用 Pt-Fe₃O₄ 中存在的协同效应来提高 NPs 对 H₂O₂ 的还原能力，从而提高了免疫传感器的灵敏度。氮掺杂石墨烯片 (N-GS) 通过在氨中对氧化石墨进行热退火从氧化石墨合成，用于固定原发性抗 SCC 抗体 (Ab₁)。二级抗 SCC 抗体 (Ab₂) 被吸附到 Pt-Fe₃O₄ NPs 上。通过三明治结构制备了免疫传感器，并显示出较宽的线性范围 (0.05-18 ng/mL)、较低的检测限 (15.3 pg/mL)、良好的重现性和稳定性。该方法已应用于临床血清样本的分析，结果令人满意。这些免疫传感器的标记物可为不同生物分子的检测提供许多潜在的应用。
