Department of Chemistry, Shanxi Normal University, Linfen, 041004, People's Republic of China.
Mikrochim Acta. 2018 Feb 8;185(3):163. doi: 10.1007/s00604-018-2695-8.
The authors have developed a homogeneous "off-on" fluorometric method for the determination of the antibiotic sulfamethazine (SMZ). Aptamer against SMZ was labeled with graphene oxide quantum dots upon which the Graphene oxide quenched the blue fluorescence of the GOQDs. On addition of SMZ, the aptamers will bind SMZ and this will cause the release of GOQDs. As a result, fluorescence will be regenerated. Fluorescence, best measured at excitation/emission wavelengths of 365/455 nm, increases linearly in the 8 pg·mL to 60 ng·mL SMZ concentration range, with a 5 pg·mL detection limit. The method is reliable and was successfully applied to the determination of SMZ in spiked milk samples, with recoveries ranging from 89 to 96% depending on analyte concentration. Graphical abstract Graphene oxide quantum dots (GOQDs) were covalently bound to the aptamer (apt) against sulfamethazine (SMZ) and adsorbed on the surface of graphene oxide (GO). This results in quenching of the fluorescence of GOQDs. On addition of SMZ, fluorescence is restored due to the release of GOQD@apt from GO.
作者开发了一种用于测定抗生素磺胺甲恶唑（SMZ）的均相“开-关”荧光法。针对 SMZ 的适体在氧化石墨烯量子点上进行标记，而氧化石墨烯会使 GOQDs 的蓝色荧光猝灭。加入 SMZ 后，适体将与 SMZ 结合，这将导致 GOQDs 的释放。结果，荧光将被再生。荧光最好在激发/发射波长为 365/455nm 处测量，在 8pg·mL 至 60ng·mL 的 SMZ 浓度范围内呈线性增加，检测限为 5pg·mL。该方法可靠，成功应用于加标牛奶样品中 SMZ 的测定，回收率取决于分析物浓度，范围为 89%至 96%。
