School of pharmacy, Nanjing medical university, Nanjing, Jiangsu, 211166, People's Republic of China.
Mikrochim Acta. 2018 Jan 29;185(2):144. doi: 10.1007/s00604-018-2683-z.
The authors describe a fluorometric aptamer based assay for adenosine triphosphate (ATP). It is based on the use of carbon dots (CDs) and graphene oxide (GO). The resultant CD-aptamer is adsorbed on the surface of GO via π-stacking and hydrophobic interaction, and the fluorescence of CD-aptamer is quenched via fluorescence resonance energy transfer (FRET) between CDs and GO. If ATP is present, it will bind to the aptamer and the CD-aptamer will be desorbed from GO. This will suppress FRET and the fluorescence of the CDs is restored. Under the optimal conditions and at typical excitation/emission wavelengths of 358/455 nm, the assay has a 80 pM detection limit and a linear range that extends from 0.10 to 5.0 nM concentrations of ATP. The method was successfully applied to the determination of ATP in yogurt samples. This method can also be conceivably applied to the detection of other analytes for which appropriate aptamers are available. Graphical abstract Schematic of a novel fluorometric ATP assay based on the fluorescence resonance energy transfer (FRET) between aptamer modified carbon dots (CD-aptamer) and graphene oxide (GO). CD-aptamer was used as the energy donor and molecular recognition probe, and GO acted as energy acceptor. This assay exhibits high sensitivity and selectivity with a detection limit as low as 80 pM.
作者描述了一种基于荧光适体的三磷酸腺苷（ATP）分析方法。它基于使用碳点（CDs）和氧化石墨烯（GO）。所得的 CD-适体通过π-堆积和疏水相互作用吸附在 GO 表面，CD-适体的荧光通过 CDs 和 GO 之间的荧光共振能量转移（FRET）猝灭。如果存在 ATP，它将与适体结合，CD-适体将从 GO 上解吸。这将抑制 FRET，恢复 CDs 的荧光。在最佳条件下，在典型的激发/发射波长为 358/455nm 时，该分析方法的检测限为 80pM，线性范围从 0.10 到 5.0nM 的 ATP 浓度。该方法成功应用于酸奶样品中 ATP 的测定。这种方法也可以应用于检测其他具有合适适体的分析物。 图表摘要 基于适配体修饰的碳点（CD-适体）和氧化石墨烯（GO）之间的荧光共振能量转移（FRET）构建新型荧光 ATP 分析方法的示意图。CD-适体用作能量供体和分子识别探针，GO 作为能量受体。该分析方法具有高灵敏度和选择性，检测限低至 80pM。
