Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
ACS Appl Mater Interfaces. 2016 Jul 20;8(28):17745-50. doi: 10.1021/acsami.6b04171. Epub 2016 Jul 5.
We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants.
我们描述了一种新的策略，用于使用化学发光共振能量转移（CRET），通过在竞争免疫分析中使用半抗原功能化的量子点（QDs）来检测磺胺甲恶唑（SMZ）。我们合成了核/多壳 QDs，并进行了磷脂-PEG 修饰。修饰后的 QDs 与半抗原 4-（4-氨基苯基-磺酰胺基）丁酸酸功能化。基于 CRET 的免疫分析对 SMZ 的检测限为 9 pg mL(-1)，比均相荧光偏振免疫分析高 4 个数量级，比异质酶联免疫吸附测定高 2 个数量级。该策略代表了一种简单、可靠、通用的检测化学污染物的方法。
