基于半抗原功能化量子点的化学发光共振能量转移竞争免疫法检测磺胺甲噁唑
Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine.
机构信息
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
出版信息
ACS Appl Mater Interfaces. 2016 Jul 20;8(28):17745-50. doi: 10.1021/acsami.6b04171. Epub 2016 Jul 5.
We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants.
我们描述了一种新的策略，用于使用化学发光共振能量转移（CRET），通过在竞争免疫分析中使用半抗原功能化的量子点（QDs）来检测磺胺甲恶唑（SMZ）。我们合成了核/多壳 QDs，并进行了磷脂-PEG 修饰。修饰后的 QDs 与半抗原 4-（4-氨基苯基-磺酰胺基）丁酸酸功能化。基于 CRET 的免疫分析对 SMZ 的检测限为 9 pg mL(-1)，比均相荧光偏振免疫分析高 4 个数量级，比异质酶联免疫吸附测定高 2 个数量级。该策略代表了一种简单、可靠、通用的检测化学污染物的方法。
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