Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Mikrochim Acta. 2018 Mar 14;185(4):222. doi: 10.1007/s00604-018-2762-1.
A method is described for rapid (1 h) synthesis of gold nanoclusters (AuNCs) co-functionalized with serine and 11-mercaptoundecanoic acid. The co-functionalized AuNCs exhibit good stability towards temperature, pH values, and over time. They were characterized by atomic force microscopy, high-resolution transmission electron microscopy, dynamic light scattering, and by fluorescence, IR and X-ray photoelectron spectroscopies. The fluorescence of the AuNCs is quenched by Hg(II) and restored on subsequent addition of aureomycin (CTC) or oxytetracycline (OTC). A fluorescent turn-on assay was worked out for simultaneous detection of CTC and OTC based on recording the change of the restored fluorescence measured at 420 and 500 nm under 340 nm photoexcitation. The detection limits are 20 and 9 nM for CTC and OTC, respectively. The concentrations of CTC and OTC can also be visualized by UV illumination. The nanoprobe was successfully applied to the simultaneous determination of CTC and OTC in spiked human urine. Graphical abstract Schematic of a two-wavelength fluorescence recovery method for the simultaneous determination of aureomycin and oxytetracycline. It is based on the use of gold nanocrystals modified with serine and 11-mercaptoundecanoic acid, and on recording the change of the restored fluorescence measured at 420 and 500 nm.
一种快速（1 小时）合成同时带有丝氨酸和 11-巯基十一酸官能团的金纳米簇（AuNCs）的方法。共功能化的 AuNCs 在温度、pH 值和时间方面表现出良好的稳定性。通过原子力显微镜、高分辨率透射电子显微镜、动态光散射以及荧光、红外和 X 射线光电子能谱对其进行了表征。Hg(II) 可以猝灭 AuNCs 的荧光，而金霉素（CTC）或土霉素（OTC）的加入可以使其恢复。基于在 340nm 光激发下测量的 420nm 和 500nm 处恢复荧光的变化，开发了一种用于同时检测 CTC 和 OTC 的荧光开启测定法。CTC 和 OTC 的检测限分别为 20 和 9nM。CTC 和 OTC 的浓度也可以通过紫外光照射可视化。该纳米探针成功应用于人尿液中 CTC 和 OTC 的同时测定。
