Key Laboratory of Sensor Anyalsis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Department of Neurology, Qingdao City Center Hospital, No.127 Siliu South Road, Qingdao, Shandong, 266042, People's Republic of China.
Mikrochim Acta. 2018 Feb 27;185(3):198. doi: 10.1007/s00604-018-2734-5.
Gold nanoclusters (AuNCs) protected with a bovine serum albumin (BSA) coating are known to emit red fluorescence (peaking at 650 nm) on photoexcitation with ultraviolet light (365 nm). On addition of Cu(II) ions, fluorescence is quenched because Cu(II) complexes certain amino acid units in the BSA chain. Fluorescence is, however, restored if pyrophosphate (PPi) is added because it will chelate Cu(II) and remove it from the BSA coating on the AuNCs. Because PPi is involved in the function of telomerase, the BSA@AuNCs loaded with Cu(II) can act as a fluorescent probe for determination of the activity of telomerase. A fluorescent assay was worked out for telomerase that is highly sensitive and has a wide linear range (10 nU to 10 fM per mL). The fluorescent probe was applied to the determination of telomerase activity in cervix carcinoma cells via imaging. It is shown that tumor cells can be well distinguished from normal cells by monitoring the differences in intracellular telomerase activity. Graphical abstract Gold nanoclusters (AuNCs) protected by bovine serum albumin (BSA) and displaying red photoluminescence were prepared as fluorescent probe for the determination of telomerase activity and used for imaging of cervix carcinoma (HeLa) cells.
金纳米簇（AuNCs）被牛血清白蛋白（BSA）保护，在紫外光（365nm）激发下会发出红色荧光（峰值在 650nm）。加入 Cu(II)离子后，荧光会被猝灭，因为 Cu(II)会与 BSA 链中的某些氨基酸单元结合。然而，如果加入焦磷酸（PPi），荧光会恢复，因为它会螯合 Cu(II)并将其从 AuNCs 上的 BSA 涂层中去除。由于 PPi 参与端粒酶的功能，负载 Cu(II)的 BSA@AuNCs 可以作为端粒酶活性的荧光探针。建立了一种用于端粒酶的荧光测定法，该方法具有高灵敏度和宽线性范围（每毫升 10nU 至 10fM）。该荧光探针通过成像应用于宫颈癌细胞中端粒酶活性的测定。结果表明，通过监测细胞内端粒酶活性的差异，可以很好地区分肿瘤细胞和正常细胞。
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