Hosseini Morteza, Mehrabi Fatemeh, Ganjali Mohammad Reza, Norouzi Parviz
Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran.
Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran.
Luminescence. 2016 Nov;31(7):1339-1343. doi: 10.1002/bio.3112. Epub 2016 Feb 22.
A fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer-templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA-AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as-prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA-AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd.
基于DNA适配体模板化银纳米簇（AgNCs）的荧光猝灭，提出了一种用于检测土霉素（OTC）的荧光适配体传感器。使用了具有两个不同核苷酸片段的特定DNA支架：一个富含胞嘧啶序列片段（C12），可通过化学还原法产生DNA-AgNCs；另一个是可与OTC抗生素选择性结合的OTC适配体片段。因此，制备的AgNCs在与目标OTC结合后会表现出荧光猝灭。DNA-AgNCs的荧光比率在0.5 nM至100 nM的范围内与目标浓度呈线性比例猝灭，检测限为0.1 nM。该新型纳米生物传感器被证明具有灵敏、选择性好和操作简单的特点，为快速测定蜂蜜和水样中的毒素OTC提供了一种可行的替代方法。版权所有© 2016约翰威立父子有限公司。
