Helmholtz Zentrum München, GmbH, German Research Center for Environmental Health, Research Unit Microbiome Analysis, Ingolstädter Landstraße 1, D-85764, Neuherberg, Germany.
Humboldt University, Berlin, Germany.
Environ Sci Pollut Res Int. 2018 Jun;25(16):16080-16090. doi: 10.1007/s11356-018-1839-z. Epub 2018 Mar 28.
Phytoremediation has been proposed to reduce the load of the sunscreen oxybenzone (OBZ) in the aquatic environment. Despite the proven removal efficiency of this compound, little is known about its influence, particularly oxidative stress on plants. In this study, a short-term incubation of macrophytic Cyperus alternifolius was performed to prove the plant's ability to withstand the stress. Detached shoots were immersed in medium spiked with different concentrations of OBZ (50, 100, and 500 μM) for 2, 4, and 7 days, respectively. Increased formation of O and HO in Cyperus treated with OBZ was characterized by intense colorization following histochemical staining. Alterations of enzyme activities involved in the antioxidative defense system indicate an adaptive response of C. alternifolius to this xenobiotic stress. Quantification of lipid peroxidation reveals that no significant membrane damage occurred during incubation with OBZ. Overall, 50 μM OBZ (tenfold higher than the amount frequently detected in the environment) exhibited low toxic effects. Accordingly, this pilot study provides information on the potential use of Cyperus to remove emerging sunscreen contaminants from water bodies.
植物修复被提议用于减少水生环境中防晒霜中氧苯酮（OBZ）的负荷。尽管已经证明了这种化合物的去除效率，但对于其影响，特别是对植物的氧化应激知之甚少。在这项研究中，对大型水生植物香蒲进行了短期培养，以证明植物承受压力的能力。将离体的嫩枝分别浸入含有不同浓度 OBZ（50、100 和 500 μM）的培养基中 2、4 和 7 天。用 OBZ 处理的香蒲中 O 和 HO 的形成增加，这可以通过组织化学染色后的强烈着色来表征。参与抗氧化防御系统的酶活性的改变表明，香蒲对这种外来生物胁迫有适应性反应。脂质过氧化产物的定量表明，在与 OBZ 孵育过程中没有发生明显的膜损伤。总的来说，50 μM OBZ（比环境中经常检测到的浓度高十倍）表现出低毒性作用。因此，这项初步研究提供了有关利用香蒲从水体中去除新兴防晒霜污染物的信息。
