脱矿牙本质基质骨诱导及相关细胞鉴定的实验研究
[Experimental study of demineralized dentin matrix on osteoinduction and related cells identification].
作者信息
Shengyin Yang, Ping Chen, Jibo Bao, Yixin Ding, Jinyang Zou, Zhigang Xie
机构信息
First Clinic, Affiliated Stomatology Hospital, Kunming Medical University, Kunming 650101, China.
Honghe Health Vocational College, Mengzi 661100, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2018 Feb 1;36(1):33-38. doi: 10.7518/hxkq.2018.01.007.
OBJECTIVE
The aim of this study was to explore the theoretical framework of cells and the forms of osteogenesis in the mechanism by which demineralized dentin matrix (DDM) induces osteogenesis.
METHODS
A total of 24 New Zealand rabbits were used in this study. A total of 4 erector spinae bags were created in each animal. A total of 3 erector spinae bags were implanted with DDM by random selection, whereas the remaining one erector spinae bag was not implanted with DDM. The rabbits were sacrificed after 1, 2, 3, 4, 8, 12, 16, and 20 weeks, and the samples were obtained. The samples were examined by hematoxylin-eosin (HE), tartrate-resistant acid phosphatase (TRAP), and immunohistochemical staining to identify the mesenchymal stem cells, osteoblasts, chondrocytes, and osteoclasts.
RESULTS: The results of HE staining showed that in the third week, cartilage- and bone-like matrices, as well as the osteoblast-like cells, were observed. The results of immunohistochemical staining showed that the expressions of CD44, alkaline phosphatase (ALP), and collagen Ⅱ were statistically significant (P<0.05).
CONCLUSIONS
DDM has good histocompatibility and osteoinduction. In addition, induced ectopic osteogenesis mode mainly occurs in the endochondral bone.
目的
本研究旨在探讨脱矿牙本质基质（DDM）诱导成骨机制中的细胞理论框架和成骨形式。
方法
本研究共使用24只新西兰兔。每只动物创建4个竖脊肌袋。随机选择3个竖脊肌袋植入DDM，而其余1个竖脊肌袋不植入DDM。在1、2、3、4、8、12、16和20周后处死兔子并获取样本。通过苏木精-伊红（HE）、抗酒石酸酸性磷酸酶（TRAP）和免疫组织化学染色检查样本，以鉴定间充质干细胞、成骨细胞、软骨细胞和破骨细胞。
结果
HE染色结果显示，在第3周时，观察到软骨样和骨样基质以及成骨样细胞。免疫组织化学染色结果显示，CD44、碱性磷酸酶（ALP）和Ⅱ型胶原的表达具有统计学意义（P<0.05）。
结论
DDM具有良好的组织相容性和骨诱导性。此外，诱导异位成骨模式主要发生在软骨内成骨。
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