Kim Seok-Kon, Huh Chang-Kyu, Lee Jae-Hoon, Kim Kyung-Wook, Kim Moon-Young
Department of Pain and Anesthesiology, College of Medicine, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam Korea.
Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam Korea.
Maxillofac Plast Reconstr Surg. 2016 Feb 13;38(1):7. doi: 10.1186/s40902-016-0053-5. eCollection 2016 Dec.
This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from human and polydeoxyribonucleotide (PDRN) for nude mice.
Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human and PDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice were sacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson's trichrome staining. The specimens were also evaluated via a histomorphometric study.
The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findings showed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed in the histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks compared with the first week and fourth week.
In this study, we showed that the PDRN used in this experimental model was able to induce bone regeneration when combined to the DDM.
本研究检测了人脱矿牙本质基质（DDM）和聚脱氧核糖核苷酸（PDRN）对裸鼠的骨诱导活性。
选用20只体重约15~20 g的健康裸鼠进行研究。制备人DDM和PDRN并皮下植入裸鼠背部。在移植后1、2和4周处死裸鼠，通过苏木精-伊红染色和马松三色染色进行组织学评估。标本还通过组织形态计量学研究进行评估。
DDM和PDRN在软组织中诱导了新骨、成骨细胞和成纤维细胞。组织学结果显示在1、2和4周时出现了成骨细胞和成纤维细胞等骨形成细胞。在组织形态计量学研究中观察到了新骨形成。特别是，与第一周和第四周相比，新骨形成率在第2周时最高。
在本研究中，我们表明在该实验模型中使用的PDRN与DDM联合使用时能够诱导骨再生。
