Li Zhong, Niu Yuanjie
Department of Urology, the Second Hospital of Tianjin Medical University, Tianjin, China.
Department of Urology, the Second Hospital of Tianjin Medical University, Tianjin, China -
Minerva Urol Nefrol. 2019 Apr;71(2):161-167. doi: 10.23736/S0393-2249.18.03004-7. Epub 2018 Mar 28.
The Long non-coding RNA (lncRNA) H19 is involved in carcinogenesis, progression, and metastasis. However, the association between genetic variants in H19 and bladder cancer susceptibility has not been reported. This case-control study assessed the association between H19 genetic variants and susceptibility to bladder cancer in a Chinese Han population.
In this study, 200 patients with bladder cancer and 200 healthy controls were surveyed and compared for frequencies of the genotypes of the H19 gene. Logistic regression analysis and the chi-square test were employed as statistical methods. Odds ratio (OR) and its corresponding 95% confidence interval (95% CI) were calculated to estimate the strength of association between H19 polymorphisms and risk of bladder cancer.
We analyzed the frequency of three lncRNA H19 SNPs in 200 bladder cancer patients and 200 healthy controls. Carriers of variant rs217727 heterozygous genotype showed increased bladder cancer risk (P=0.008). Further stratified analyses revealed that the association between bladder cancer risk and variant genotypes of rs217727 was more profound in smokers. Furthermore, decreased risk of invasive bladder cancer was found in carrying rs3024270 CC patients.
Our results provided the evidence that rs217727 in H19 was associated with elevated risk of bladder cancer, which may be a potential biomarker for predicting bladder cancer susceptibility. Furthermore, invasive bladder cancer in carrying rs3024270 CC genotype maybe have a good prognosis.
长链非编码RNA（lncRNA）H19参与肿瘤发生、进展和转移。然而，H19基因变异与膀胱癌易感性之间的关联尚未见报道。本病例对照研究评估了中国汉族人群中H19基因变异与膀胱癌易感性之间的关联。
本研究对200例膀胱癌患者和200例健康对照进行调查，比较H19基因各基因型的频率。采用逻辑回归分析和卡方检验作为统计方法。计算比值比（OR）及其相应的95%置信区间（95%CI），以评估H19基因多态性与膀胱癌风险之间的关联强度。
我们分析了200例膀胱癌患者和200例健康对照中三个lncRNA H19单核苷酸多态性（SNP）的频率。rs217727变异杂合基因型携带者的膀胱癌风险增加（P=0.008）。进一步的分层分析显示，rs217727变异基因型与膀胱癌风险之间的关联在吸烟者中更为显著。此外，携带rs3024270 CC基因型的患者浸润性膀胱癌风险降低。
我们的结果提供了证据，表明H19基因中的rs217727与膀胱癌风险升高相关，这可能是预测膀胱癌易感性的一个潜在生物标志物。此外，携带rs3024270 CC基因型的浸润性膀胱癌可能预后良好。
