Zhang Hao, Zhao Fangfang, Peng Along, Dong Li, Wang Mengzhi, Yu Lihuai, Loor Juan J, Wang Hongrong
Department of Animal Sciences and Division of Nutritional Sciences , University of Illinois , Urbana , Illinois 61801 , United States.
J Agric Food Chem. 2018 Apr 25;66(16):4145-4154. doi: 10.1021/acs.jafc.8b00726. Epub 2018 Apr 10.
This study investigated the effects of dietary l-arginine (Arg) and N-carbamylglutamate (NCG) supplementation on intestinal integrity, immune function, and oxidative status in intrauterine-growth-retarded (IUGR) suckling lambs. A total of 48 newborn Hu lambs of normal birth weight (CON) and IUGR were allocated randomly into four groups of 12 animals each: CON, IUGR, IUGR + 1% Arg, or IUGR + 0.1% NCG. All lambs were raised for a period of 21 days from 7 to 28 days after birth. The Arg or NCG group exhibited improved ( p < 0.05) final body weights compared to that of the IUGR group. In comparison to the IUGR lambs, the apoptotic percentage was lower ( p < 0.05) in the ileum of IUGR lambs supplemented with Arg and NCG. In addition, in comparison to IUGR, the concentrations of protein carbonyl and malondialdehyde were lower ( p < 0.05) and the reduced glutathione (GSH) concentration and ratio of GSH/oxidized glutathione were greater ( p < 0.05) in the jejunum, duodenum, and ileum of IUGR + 1% Arg or 0.1% NCG lambs. In comparison to the IUGR group, the mRNA abundance of myeloid differentiation factor 88, toll-like receptor 9, toll-like receptor 4, interleukin 6, and fuclear factor-κB was lower ( p < 0.05) and the mRNA abundance of superoxide dismutase 1, B-cell lymphoma/leukaemia 2, zonula occludens-1 (ZO-1), and occludin was greater in the ileum of the IUGR lambs supplemented with Arg or NCG. Furthermore, the protein abundance of ZO-1 and claudin-1 in the ileum was greater ( p < 0.05) in the IUGR + 1% Arg or 0.1% NCG lambs. The results show that Arg or NCG supplementation improves the growth, intestinal integrity, immune function, and oxidative status in IUGR Hu suckling lambs.
本研究调查了日粮添加L-精氨酸（Arg）和N-氨甲酰谷氨酸（NCG）对宫内生长受限（IUGR）哺乳羔羊肠道完整性、免疫功能和氧化状态的影响。将48只正常出生体重的新生湖羊羔羊（CON）和IUGR羔羊随机分为四组，每组12只动物：CON组、IUGR组、IUGR + 1% Arg组或IUGR + 0.1% NCG组。所有羔羊在出生后7至28天饲养21天。与IUGR组相比，Arg或NCG组的最终体重有所改善（p < 0.05）。与IUGR羔羊相比，添加Arg和NCG的IUGR羔羊回肠中的凋亡百分比更低（p < 0.05）。此外，与IUGR相比，IUGR + 1% Arg或0.1% NCG羔羊的空肠、十二指肠和回肠中蛋白质羰基和丙二醛的浓度更低（p < 0.05），还原型谷胱甘肽（GSH）浓度以及GSH/氧化型谷胱甘肽的比值更高（p < 0.05）。与IUGR组相比，添加Arg或NCG的IUGR羔羊回肠中髓样分化因子88、Toll样受体9、Toll样受体4、白细胞介素6和核因子-κB的mRNA丰度更低（p < 0.05），超氧化物歧化酶1、B细胞淋巴瘤/白血病2、紧密连接蛋白-1（ZO-1）和闭合蛋白的mRNA丰度更高。此外，IUGR + 1% Arg或0.1% NCG羔羊回肠中ZO-1和claudin-1的蛋白质丰度更高（p < 0.05）。结果表明，添加Arg或NCG可改善IUGR湖羊哺乳羔羊的生长、肠道完整性、免疫功能和氧化状态。
