Hu Naizhi, Mao Pei, Xiong Xiaoya, Ma Zhuangzhuang, Xie Zhijiang, Gao Mengmeng, Wu Qiujue, Ma Wenfeng
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China.
Animals (Basel). 2023 Oct 12;13(20):3183. doi: 10.3390/ani13203183.
Weaning is an important period that affects the performance of piglets. However, the regulation of dietary amino acid levels is considered to be an effective way to alleviate the weaning stress of piglets. N-carbamylglutamate (NCG) plays an important role in improving the growth performance and antioxidant capacity of animals. A total of 36 weaned piglets were randomly assigned to two treatment groups, a control group (CON) and a 500 mg/kg NCG group (NCG), and the experiment lasted for 28 days. The results show that the NCG treatment group showed an increased 0-28 days average weight gain and average daily feed intake, and also increased contents of GLU and HDL, and lower SUN in serum, and an upregulation of the expression of the amino acid transporters SNAT2, EAAC1, SLC3A1, and SLC3A2 mRNA in the jejunum ( < 0.05), as well as an increased villus length and VH:CD ratio, and claudin-1, occludin, and ZO-1 mRNA expression in the jejunum ( < 0.05). The NCG treatment group showed an increased content of GSH-Px in serum and T-AOC and SOD in the jejunum, and a lower content of MDA ( < 0.05); and the upregulation of the mRNA expression related to antioxidant enzymes (CAT, SOD1, Gpx4, GCLC, GCLM and Nrf2, AhR, CYP1A1) in the jejunal mucosa ( < 0.05). In addition, compared with the control group, the NCG treatment group saw an upregulation in the mRNA expression of IL-10 and a decrease in the expression of IL-1β and IL-4 in the jejunal mucosa ( < 0.05). In summary, the results of this study suggest that NCG improved growth performance and jejunal morphology, improved the jejunal transport of amino acids related to the ornithine cycle, and improved the antioxidant capacity in weaned pigs.
断奶是影响仔猪生长性能的重要时期。然而，调节日粮氨基酸水平被认为是缓解仔猪断奶应激的有效途径。N-氨甲酰谷氨酸（NCG）在提高动物生长性能和抗氧化能力方面发挥着重要作用。将36头断奶仔猪随机分为两个处理组，即对照组（CON）和500 mg/kg NCG组（NCG），试验持续28天。结果表明，NCG处理组0至28天的平均体重增加和平均日采食量增加，血清中GLU和HDL含量增加，SUN降低，空肠中氨基酸转运体SNAT2、EAAC1、SLC3A1和SLC3A2 mRNA的表达上调（P<0.05），同时空肠绒毛长度和VH:CD比值增加，claudin-1、occludin和ZO-1 mRNA表达上调（P<0.05）。NCG处理组血清中GSH-Px含量增加，空肠中T-AOC和SOD增加，MDA含量降低（P<0.05）；空肠黏膜中抗氧化酶（CAT、SOD1、Gpx4、GCLC、GCLM和Nrf2、AhR、CYP1A1）相关的mRNA表达上调（P<0.05）。此外，与对照组相比，NCG处理组空肠黏膜中IL-10的mRNA表达上调，IL-1β和IL-4的表达降低（P<0.05）。综上所述，本研究结果表明，NCG改善了断奶仔猪的生长性能和空肠形态，改善了与鸟氨酸循环相关的氨基酸在空肠中的转运，并提高了抗氧化能力。
