Thiele D J, Wright C F, Walling M J, Hamer D H
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health.
Experientia Suppl. 1987;52:423-9. doi: 10.1007/978-3-0348-6784-9_41.
A functional copperthionein (CUP1) gene in Saccharomyces cerevisiae is essential to prevent copper-mediated cytotoxicity, but is dispensable for cell growth in the absence of exogenous copper. The CUP1 gene is negatively autoregulated, as observed by the necessity for a functional CUP1 gene in order to repress basal level transcription from the CUP1 promoter. Both the copper protection and transcriptional autoregulatory functions can be complemented by expression in yeast of either of two monkey metallothionein isoform cDNAs. The expression of the CUP1 gene is induced at the level of transcription by copper via cis-dominant upstream control sequences which are tandemly repeated. Synthetic CUP1 upstream control sequences confer copper inducibility on a heterologous yeast promoter in a manner similar to that observed for the authentic upstream control region.
酿酒酵母中的功能性铜硫蛋白（CUP1）基因对于预防铜介导的细胞毒性至关重要，但在没有外源铜的情况下对细胞生长并非必需。如通过功能性CUP1基因抑制CUP1启动子基础水平转录的必要性所观察到的，CUP1基因存在负自调控。两种猴金属硫蛋白同工型cDNA中的任何一种在酵母中的表达都可以补充铜保护和转录自调控功能。CUP1基因的表达通过串联重复的顺式显性上游控制序列在转录水平上由铜诱导。合成的CUP1上游控制序列以类似于真实上游控制区域的方式赋予异源酵母启动子铜诱导性。
