Hamer D H, Thiele D J, Lemontt J E
Science. 1985 May 10;228(4700):685-90. doi: 10.1126/science.3887570.
The CUP1 gene of yeast encodes a small, metallothionein-like protein that binds to and is inducible by copper. A gene replacement experiment shows that this protein protects cells against copper poisoning but is dispensable for normal cellular growth and development throughout the yeast life cycle. The transcription of CUP1 is negatively autoregulated. This feedback mechanism, which is mediated through upstream control sequences, may play an important role in heavy metal homeostasis.
酵母的CUP1基因编码一种小的、类金属硫蛋白的蛋白质，该蛋白质能与铜结合并受铜诱导。一项基因替换实验表明，这种蛋白质可保护细胞免受铜中毒，但在酵母整个生命周期的正常细胞生长和发育过程中并非必需。CUP1的转录受到负向自我调节。这种通过上游控制序列介导的反馈机制可能在重金属稳态中发挥重要作用。
