金属硫蛋白在过渡金属解毒及耐受性中的作用。

Role of metallothionein in detoxification and tolerance to transition metals.

作者信息

Huang P C, Morris S, Dinman J, Pine R, Smith B

机构信息

Department of Biochemistry, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205.

出版信息

Experientia Suppl. 1987;52:439-46. doi: 10.1007/978-3-0348-6784-9_43.

DOI:10.1007/978-3-0348-6784-9_43

PMID:2959533

Abstract

Animal tolerance to the transition metals cadmium and zinc is hereditary. The evolution to a high level of resistance can be accelerated through mutation and selective pressure. We have studied inbred strains of mice and mutants of Chinese hamster ovary cells resistant to cadmium to further these understandings. Results with whole animals show that the difference in the rate and level of metallothionein accumulation is at most twofold between sensitive and resistant strains. However, with cadmium resistant CHO mutant cells, there is an over 60 fold increase in metallothionein and its mRNA upon induction. These mutants show over 60 fold amplification in metallothionein genes. These results offer a direct contrast in the correlation between elevation of metal resistance and increases in metallothionein between two genetic systems.

摘要

动物对过渡金属镉和锌的耐受性具有遗传性。通过突变和选择压力，可以加速向高水平抗性的进化。我们研究了对镉具有抗性的近交系小鼠和中国仓鼠卵巢细胞突变体，以进一步深化这些认识。对完整动物的研究结果表明，敏感品系和抗性品系之间金属硫蛋白积累的速率和水平差异至多为两倍。然而，对于对镉具有抗性的中国仓鼠卵巢（CHO）突变体细胞，诱导后金属硫蛋白及其信使核糖核酸（mRNA）增加了60多倍。这些突变体的金属硫蛋白基因扩增了60多倍。这些结果直接对比了两个遗传系统中金属抗性升高与金属硫蛋白增加之间的相关性。